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OCT 20, 2021 9:00 AM PDT

Membrane Proteins: From gene to cryo-EM structure with Thermo Fisher GeneArt and Salipro Biotech

Speakers

Abstract

Date: October 20, 2021

Time: 9:00am PDT, 12:00pm EDT

 

Cryo-electron microscopy (cryo-EM) is rapidly evolving to be the primary tool for structure determination of membrane proteins. However, generation of high quality membrane protein for structural biology can be challenging and is often a bottleneck. In this webinar, we demonstrate that the combined expertise of Thermo Fisher Scientific and Salipro Biotech can be leveraged for the Gene-to-Structure generation of membrane protein drug targets. Starting with the gene - the GeneArt platform comprises a Gene-to-Protein service that only requires a protein sequence and covers every step from gene synthesis to protein purification, involving Thermo Fisher’s proprietary mammalian and insect expression systems - Expi293, ExpiCHO or ExpiSF9. These cell lines form an excellent basis for membrane protein overexpression. Since GeneArt focuses on the purification of soluble intracellular and secreted proteins, we collaborated with Salipro Biotech for expertise in membrane proteins. Salipro Biotech has developed a proprietary nano-membrane platform technology (Salipro®) that stabilizes all types of integral membrane proteins in their native forms in lipid environments, enabling them to be employed in drug discovery programs for therapeutic antibodies, small molecule drugs and structure-based drug design. With the DirectMX® methodology, Salipro Biotech has developed a one-step approach that allows for the direct purification and reconstitution of membrane proteins straight from crude cells. Combining Thermo Fisher Scientific and the Salipro® platform technologies can therefore overcome one of the biggest barriers of the cryo-EM workflow. Since the presented workflow yields and validates high-quality proteins, it benefits a wide range of downstream workflows, ranging from structure-based drug design to the development of therapeutic antibodies.

 

Learning Objectives

  • Discuss the value of GeneArt mammalian and insect expressions for cryo-EM.
  • Describe Salipro Biotech Direct Mx technology for purification and stabilization of membrane proteins.
  • Discuss Cryo-EM for structure determination of membrane proteins.

 

 

Webinars will be available for unlimited on-demand viewing after live event.

 

LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

 


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