MENU
NOV 14, 2018 3:00 PM PST

Modeling Inflammation and Fibrosis in Humans with PSC-derived Steatohepatitis Liver Organoids

Speaker
  • Associate Director, CuSTOM, Professor and Assistant Professor, Cincinnati Children's Hospital Tokyo Medical and Dental University Yokohama City University, Principal Investigator, Takeda-CiRA
    Biography
      Dr. Takebe is an Associate Director of Center for Stem Cell and Organoid Medicine (CuSTOM) at the Cincinnati Children's Hospital Medical Center and Professor at Yokohama City University, and Tokyo Medical and Dental University, Japan. He serves as Board of Directors at International Society of Stem Cell Research (ISSCR). His lab investigates the mechanisms of human organogenesis, and develops mini-organ technologies from human stem cells - namely organ bud based approaches. He is applying iPSC-liver buds into drug discovery study as well as transplant application - for patients with a rare congenital metabolic disorder, ultimately expanding the clinical indications to diseases like liver cirrhosis.

    Abstract

    Human organoid systems recapitulate in vivo organ architecture, yet fail to capture complex pathologies such as inflammation and fibrosis. Here we developed multi-cellular human liver organoids (HLO) from 11 different healthy and diseased PSC lines that display essential features of steatohepatitis. Single-cell-level analysis revealed steatohepatitis organoids exhibited persistent hepatic steatosis, followed by progressive activation of pro-inflammatory and fibrotic lineages. Interestingly, expression of the steatohepatitis phenotype correlates with the presence of clinically significant GWAS factors: PNPLA3, GCKR and TM6SF2. We developed an organoid-level readout with atomic force microscopy, and demonstrated that organoid stiffening reflects the fibrosis severity. Furthermore, organoid model of an iatrogenic parental nutrition associated liver disease (PNALD) identified obeticholic acid as effective in preventing pathology progression, suggesting the potential repurposing for PNALD. The presented key methodology and preliminary results offer a new method for studying human precision for inflammation and fibrosis, facilitating the discovery of effective treatments.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    MAR 18, 2021 8:00 AM PDT
    C.E. CREDITS
    MAR 18, 2021 8:00 AM PDT
    DATE: March 18, 2021 TIME: 8:00am PDT Sequencing of bulk cells, single cells, and nuclei is opening doors in the understanding of complex biological processes....
    NOV 14, 2018 3:00 PM PST

    Modeling Inflammation and Fibrosis in Humans with PSC-derived Steatohepatitis Liver Organoids


    Specialty

    Molecular Biology

    Cell Biology

    Medicine

    Cancer Research

    Clinical Research

    Genetics

    Biology

    Biotechnology

    Cell Line Development

    Diagnostics

    Assay Development

    Stem Cell Technologies

    Developmental Biology

    Cell Culture

    Pharmacology

    Geography

    North America56%

    Europe15%

    Asia12%

    Africa9%

    South America5%

    Oceania3%

    Registration Source

    Website Visitors91%

    Client/Sponsor Promotions4%

    Email Promotions1%

    Social Media Promotions1%

    WAVE Newsletter Promotion1%

    Other3%

    Job Title

    Student37%

    Research Scientist22%

    Post Doc9%

    Educator/Faculty8%

    Medical Doctor/Specialist7%

    Scientist4%

    Executive4%

    Marketing/Sales2%

    Clinical Laboratory Scientist2%

    Biologist2%

    Lab Management2%

    Facility/Department Manager2%

    Organization

    Academic Institution27%

    Medical School14%

    Research Institute12%

    Hospital10%

    Medical Center6%

    Biotech Company6%

    Government4%

    Clinical Laboratory4%

    Life Science Company3%

    Non-Profit Organization3%

    Association2%

    Pharmaceutical Company1%

    Ambulatory Care1%

    Health Plan/hmo1%

    Other4%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more