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New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells

Speaker

Abstract

The availability of well-characterised monoclonal antibodies (mAbs) detecting cell-surface epitopes on human pluripotent stem cells (hPSCs) provides useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. We recently described generation of seven new mAbs that detect cell surface proteins present on primed and naive human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. In addition, we report that subsets of the seven new mAbs are also immunoreactive to specific human somatic cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types.


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New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells



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