OCT 08, 2020 9:00 AM PDT

Blood-based detection of EGFR and KRAS mutations for NSCLC patients using multiplexed droplet digital PCR

Sponsored by: Bio-Rad Laboratories
C.E. Credits: P.A.C.E. CE Florida CE
Speaker

Abstract

Mutations in the epidermal growth factor receptor gene (EGFR) and the known Kirsten rat sarcoma viral oncogene  (KRAS) are significant drivers in the development of non-small-cell lung cancer (NSCLC). Treatment is typically guided by which mutations are present, and there are several possible mutations that can arise during treatment, so detecting them quickly and reliably is key to helping the patient. With NSCLC, biopsied patient samples tend to be very small, which can hamper many conventional genetic tests. Thus the ideal test in NSCLC is one that can work with minimal inputs, and test for several mutations at the same time. This webcast will present a new liquid biopsy test using multiplexing assays that contained mutant variants. The QX ONE Droplet Digital PCR (ddPCR) System allows for automated operation and advanced multiplexing to detect mutations in EGFR and KRAS using as little as 5 ng of cell-free DNA extracted from plasma.

Learning Objectives:

1. What are the key benefits to having a high-throughput, multiplexing ddPCR system?

2. Are the results the same between standard duplex reactions and multiplexed reactions?

3. What is the greatest number of samples that can be processed to results overnight on the QX200 vs the QX ONE?


Show Resources
You May Also Like
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 8:00 AM PDT
C.E. CREDITS
JUL 15, 2021 8:00 AM PDT
Date: July 15, 2021 Time: 8:00am (PDT), 11:00am (EDT) High dimensional full spectrum flow cytometry grants unprecedented access to previously unattainable parameters in cellular biology. Flu...
NOV 09, 2021 11:00 AM PST
C.E. CREDITS
NOV 09, 2021 11:00 AM PST
Date: November 09, 2021 Time: 11:00am (PDT), 02:00pm (EDT) Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term gro...
NOV 17, 2021 8:00 AM PST
C.E. CREDITS
NOV 17, 2021 8:00 AM PST
Date: November 17, 2021 Time: 8:00am (PDT), 11:00am (EDT) From waste disposal to promising biomarkers and therapeutic agents, exosomes and other extracellular vesicles are valuable in resear...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
Loading Comments...
Show Resources