There is increasing evidence that intracellular miRNAs play a role in the pathogenesis of many complex disease phenotypes. In addition, extracellular miRNAs in exosomes are emerging as vehicles for cell-cell communication and biomarkers of various disease phenotypes. Exosomes are extracellular vesicles involved in cell-cell communication that contain proteins and RNAs, including miRNAs. A main research focus of our laboratory is to identify and understand the role of miRNAs in cell-to-cell communication influencing gene expression, specifically in cardiac disease. As a disease model, we utilize induced-pluripotent stem cell-derived (IPSC) cardiomyocytes. For many aspects, these cells recapitulate the phenotype of a patient from whom they were generated. In this presentation, we will discuss strategies to comprehensively assay miRNA, including the downstream analysis methods for both intracellular, as well as exosome derived miRNAs. In addition, we will discuss how comprehensive expression analyses identify miRNA-mRNA relationships and pathway analyses provide insights into underlying disease pathways.