MAY 09, 2018 01:30 PM PDT

Next-Generation Sequencing to Understand the Function of miRNAs in Human IPSC-Cardiomyocyte Disease Models

C.E. CREDITS: P.A.C.E. CE | Florida CE
  • Professor of Pediatrics, Medicine and Physiology, Chief - Section of Genomic Pediatrics, Department of Pediatrics, Medical College of Wisconsin
      Ulrich Broeckel, MD is a Professor of Pediatrics, Medicine and Physiology and the Chief of the Section of Genomic Pediatrics, at the Medical College of Wisconsin. Dr. Broeckel received his MD from the University of Heidelberg, Germany. He continued his training in internal medicine and cardiology. His research focuses on genetics of cardiovascular diseases, pediatric genetics and pharmacogenetics. His lab uses induced pluripotent stem cells to study the functional effect of genome variation. He is also the technical director of a CAP/CLIA certified clinical genetic testing laboratory.
    • Research Associate I, Project Leader - miRNA Technologies, Section of Genomic Pediatrics, Department of Pediatrics, Medical College of Wisconsin
        Amy is a Research Associate at the Medical College of Wisconsin and has spent the last ten years working on both research and clinical diagnostic testing in the laboratory of Dr. Ulrich Broeckel. Her primary interest is in transnational research, in particular in the area of left ventricular hypertrophy (LVH). By combining genetic information with functional analysis utilizing hiPSC-Cardiomyocytes (NGS expression analysis, proteomics, etc.), her goal is to provide novel insights into the complex regulatory role of miRNAs, both intracellular and exosomal, in the development of cardiovascular dysfunction and how genetic variation affects this. Her other research interests are in Pharmacogenomics, TKI induced cardiotoxicity, the role of extracellular matrix on cardiovascular function in disease, and studying the impact of post translational RNA editing on protein function.


      There is increasing evidence that intracellular miRNAs play a role in the pathogenesis of many complex disease phenotypes.  In addition, extracellular miRNAs in exosomes are emerging as vehicles for cell-cell communication and biomarkers of various disease phenotypes.  Exosomes are extracellular vesicles involved in cell-cell communication that contain proteins and RNAs, including miRNAs. A main research focus of our laboratory is to identify and understand the role of miRNAs in cell-to-cell communication influencing gene expression, specifically in cardiac disease. As a disease model, we utilize induced-pluripotent stem cell-derived (IPSC) cardiomyocytes. For many aspects, these cells recapitulate the phenotype of a patient from whom they were generated.  In this presentation, we will discuss strategies to comprehensively assay miRNA, including the downstream analysis methods for both intracellular, as well as exosome derived miRNAs. In addition, we will discuss how comprehensive expression analyses identify miRNA-mRNA relationships and pathway analyses provide insights into underlying disease pathways.  

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