This two-part webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples. Part 1 will feature QIAGEN’s solutions for lysis of tough bacteria and fungi and removal of challenging enzymatic inhibitors in order to obtain sequencing-quality DNA from human stool samples. In Part 2, we will focus on QIAGEN’s novel technology for 16S and ITS sequencing. We will present data from a study showcasing the link between Body Mass Index (BMI) and the salivary.
1. Learn some common workflows for generating microbiome data
2. Understand the importance of removing inhibitors from difficult samples to ensure best possible NGS results
3. Introduction to 16S sequencing and some novel techniques to improve data quality