MENU
SEP 10, 2014 7:00 AM PDT

Physical & Chemical Characterization of Nanoparticle Constructs using the Analytical Ultracentrifuge

Speaker
  • Assistant Professor, Materials Science and Engineering, King Abdullah University of Science and Technology (KAUST), Saudi Arabia
    Biography
      Osman M. Bakr is an Assistant Professor of Materials Science and Engineering, SABIC Presidential Career Development Chair, at King Abdullah University of Science and Technology (KAUST), Saudi Arabia. He holds a B.Sc. in Materials Science and Engineering from MIT (2003) as well as a M.S. and Ph.D. in Applied Physics from Harvard University (2009). He was a post-doctoral fellow in the Laboratory for Nanoscale Optics at Harvard University. In 2010 he moved to KAUST and founded the Functional Nanomaterials Lab, a research group dedicated towards the study of nanoparticles and quantum dots; particularly advancing their synthesis and self-assembly for applications in photovoltaics, optoelectoronics, and photocatalysis.

    Abstract

     

    Colloidal nanoparticles have become important materials in biomedicine, catalysis, and optoelectronics. Their unique nanoscale properties stem from their size (particularly of the inorganic particle core) and surface composition of their organic ligand-shell. Given the polydispersity of nanoparticles, and complex composition, no technique other than Analytical Ultracentrifugation (AUC) has the potential to provide detailed information on the overall particle size, density, molecular weight and aggregation state in solution.

    While AUC has become a standard tool for bioscientists to quantitatively study the size, confirmation, and interaction of macromolecules and proteins in solution for many decades, it has not had the same success with nanoparticles. Unlike nanoparticles, most proteins have a well-known partial specific volume, and hence it’s straightforward to convert quantities measured from AUC, such as the sedimentation coefficients, into parameters that provide physical insights into the macromolecule, such as frictional ratio (i.e. shape) and molecular weight.

    Recent advancements in the mathematical modeling of the sedimentation boundaries have enabled the parameterization of the sedimentation and diffusion coefficients of all the species detected in solution during centrifugation in terms of their partial specific volume and molecular weight. This advancement opens the door to quantitative investigation of most nanoparticle systems by AUC - without a priori knowledge of their partial specific volume - since most nanoparticles have a constant shape which can be assessed by electron microscopy.

    In this tutorial webinar I will introduce some of the latest methods and procedures to analyze nanoparticles in solution with AUC. I will also provide insights on avoiding common experimental challenges that researchers face when studying nanoparticles with the AUC, which may lead to artifacts in the experimental data.

    Learning Objectives:

    • Learn about the latest methods and procedures to analyze nanoparticles in solution with Analytical Ultracentrifugation (AUC)
    • Learn insights on avoiding common experimental challenges that researchers face when studying nanoparticles with the AUC

    Show Resources
    You May Also Like
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 23, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 23, 2020 TIME: 10:00am PT Human mesenchymal stromal or stem cells (MSCs)-based immunomodulation treatment has been proposed as a suitable therapeutic approach for many diseases, s...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: June 9, 2020 TIME: 10:00am PT, 1:00pm ET The presentation will first discuss sepsis as a disease and then explain the importance of performing diagnostic tests in the clinical labora...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    APR 27, 2020 9:00 AM PDT
    C.E. CREDITS
    APR 27, 2020 9:00 AM PDT
    DATE: April 27, 2020 TIME: 9:00am PT, 12:00pm ET Recombinant adeno-associated virus (rAAV) is a small (25-nm) virus that is composed of a protein shell called a capsid and a single-stranded...
    Loading Comments...
    Show Resources
    Attendees
    • See more