DATE: October 30, 2018
TIME: 10:00am PDT, 1:00pm EDT
Identification and quantification of post-translational modifications (PTM) presents a unique challenge to proteomic studies. Often PTMs feature low abundances and many possible functionally-distinct locations may exist within each protein. Although label-free data independent acquisitions (DIA or SWATH®Acquisition) have been extensively used for global proteomics workflows and quantification of proteins, so far there are only few studies applying DIA to study post-translational modifications. Here, we present several DIA-PTM studies, specifically of phosphorylated as well as acylated proteins using different affinity enrichment approaches. We are specifically using Cell Signaling Technology affinity enrichment kits for our work (PTMScan® affinity enrichment kits for acetylation/succinylation and PTMScan® Direct Tyrosine and Serine/Threonine Kinases Reagents).
In this webinar you will learn:
Use of simultaneous affinity enrichment workflows by combining different PTM antibodies for ‘one-pot enrichment’ approaches
Relevance of assessing protein PTM isoforms based on PTM site localization
Applications involving stable isotope labeled PTM-peptides