POSTER: Pro-Detect Rapid Assay Lateral Flow Kits for Detecting Protein Tags and Antibody Isotyping

Speaker
  • Senior R & D Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Krishna's PhD and postdoctural work focused on understanding control mechanisms regulating the initiation of protein translation in eukaryotes. Following his academic training, he joined Thermo Fisher Scientific and has led multiple projects including the development of the HeLa and CHO lysate based cell-free translation systems, also known asThermo Scientific1-Step In VitroTranslation (IVT) Systems, which contain all the necessary reagents to express proteins of interest in as little as one hour.

    Abstract

    Lateral flow assays (LFAs) are rapid, sensitive, and easy-to-use immunoassays for detecting specific target proteins in vitro. Although the LFA format is extremely popular in point-of-care diagnostic tests (i.e., pregnancy tests), to-date its use in basic research laboratories is not widespread. Here we present the Thermo Scientific™ Pro-Detect Rapid Assay, an LFA designed for research workflows that can be used for rapid monitoring of expressed recombinant proteins and rapid identification of antibody isotypes. The Pro-Detect Rapid Assay is an alternative to western blotting and ELISA formats that require significantly more time for sample processing as well as additional instrumentation. The rapid qualitative (yes/no) determination of this LFA will also help address key pain points in cell culture and protein expression workflows.

    Learning Objectives:

    • Understand how lateral flow technology–based Pro-Detect Rapid Assays can be used to rapidly detect protein expression

     


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    JUL 22, 2020 10:00 AM PDT
    C.E. CREDITS
    JUL 22, 2020 10:00 AM PDT
    DATE: July 23, 2020 TIME: 10:00 am PDT The SARS-CoV-2 pandemic has taken a toll on many sectors of the medical community. As the pandemic took a grip on the laboratory, the need for diagnost...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    SEP 03, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 03, 2020 9:00 AM PDT
    DATE: September 3, 2020 TIME: 09:00am PT, 12:00pm ET xxx Learning Objectives: xxx Webinars will be available for unlimited on-demand viewing after live event. LabRoots is approved as a provi...
    MAY 12, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 12, 2020 9:00 AM PDT
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    JUN 30, 2020 9:00 AM PDT
    C.E. CREDITS
    JUN 30, 2020 9:00 AM PDT
    DATE: June 30, 2020 TIME: 9:00am PDT, 12:00pm EDT QC laboratories perform a critical role in demonstrating pharmaceutical products are consistently manufactured, safe, potent, and pure. At t...
    Loading Comments...
    Show Resources
    Attendees
    • See more