POSTER: Pro-Detect Rapid Assay Lateral Flow Kits for Detecting Protein Tags and Antibody Isotyping

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Senior R & D Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Krishna's PhD and postdoctural work focused on understanding control mechanisms regulating the initiation of protein translation in eukaryotes. Following his academic training, he joined Thermo Fisher Scientific and has led multiple projects including the development of the HeLa and CHO lysate based cell-free translation systems, also known asThermo Scientific1-Step In VitroTranslation (IVT) Systems, which contain all the necessary reagents to express proteins of interest in as little as one hour.

    Abstract:

    Lateral flow assays (LFAs) are rapid, sensitive, and easy-to-use immunoassays for detecting specific target proteins in vitro. Although the LFA format is extremely popular in point-of-care diagnostic tests (i.e., pregnancy tests), to-date its use in basic research laboratories is not widespread. Here we present the Thermo Scientific™ Pro-Detect Rapid Assay, an LFA designed for research workflows that can be used for rapid monitoring of expressed recombinant proteins and rapid identification of antibody isotypes. The Pro-Detect Rapid Assay is an alternative to western blotting and ELISA formats that require significantly more time for sample processing as well as additional instrumentation. The rapid qualitative (yes/no) determination of this LFA will also help address key pain points in cell culture and protein expression workflows.

    Learning Objectives:

    • Understand how lateral flow technology–based Pro-Detect Rapid Assays can be used to rapidly detect protein expression

     


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    Loading Comments...