WEBINAR: Protein Sample Evaluation Using the NanoDrop One UV-Vis Spectrophotometer

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Product Marketing Manager-NanoDrop instruments, Thermo Fisher Scientific
    Biography
      Voula Kodoyianni is the product manager for Thermo Scientific™ NanoDrop™ microvolume UV-Vis instruments and led the development and market launch of the NanoDrop One UV-Vis Spectrophotometer in 2015. Prior to joining Thermo Fisher Scientific, Dr. Kodoyianni was the chief scientific officer for GWC Technologies, led a genome sequencing team at the University of Wisconsin-Madison, and was a Howard Hughes Medical Institute Fellow conducting research in C. elegans development. She holds a master's degree in chemistry from Duke University and a PhD in biochemistry from the University of Wisconsin-Madison.

    Abstract:

    Protein sample evaluation is an important step in many protein workflows. The NanoDrop One microvolume spectrophotometer supports protein sample quantification with multiple preconfigured applications, including direct absorbance at A280, A205, or other wavelengths using the Thermo Scientific™ Pierce™ colorimetric assays (BCA, Rapid Gold BCA, Pierce 660, Bradford, etc.) and a proteins and labels application for quantitation of metalloproteins and fluorescently labeled proteins. We will review what you need to consider when choosing a protein quantification method for your sample. Is the sample one purified protein or a mixture of proteins? Does your peptide contain tyrosine and tryptophan residues? Does the sample buffer absorb in the UV spectrum? How can co-purified DNA affect your protein sample measurement? What is a good blank/control for your sample? Answers to these questions and other key considerations will be discussed.

    Learning Objectives:

    • Understand protein extinction coefficients—how to obtain the most accurate protein sample quantification using UV absorbance at 280 nm
    • Learn how new Thermo Scientific™ Acclaro™ sample intelligence technology in the NanoDrop One spectrophotometer delivers information not only about the concentration but also the quality of your protein sample

     


    Show Resources
    You May Also Like
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    AUG 15, 2018 08:00 AM PDT
    C.E. CREDITS
    AUG 15, 2018 08:00 AM PDT
    DATE: August 15, 2018TIME: 08:00AM PDT, 11:00AM EDTThe failure of current chemotherapeutic strategies in the fight against cancer can be largely attributed to the occurrence of drug res...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    JUN 26, 2018 06:00 AM PDT
    C.E. CREDITS
    JUN 26, 2018 06:00 AM PDT
    Date: June 26, 2018Time: 6:00 a.m. PDT, 9:00 a.m. EDT, 1500 CEST Today’s hematology analyzers employ various methods for enumerating platelets. These methods include: e...
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    Loading Comments...