DEC 10, 2019 7:00 AM PST

Proven Answers for Protein, Vaccine and Gene Therapy Analysis

Sponsored by: SCIEX
C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Senior Application Scientist, SCIEX
    Biography
      Mukesh obtained a Ph.D. in Molecular Biology after getting a Masters in Biochemistry. Mukesh has a broad background in biochemistry, bio-analytical chemistry and molecular biology. His current focus at Sciex is developing new analytical methods for Cell and Gene Therapy working with scientists at biopharmaceutical companies, as well at the universities/government research labs. Earlier, he was a core team member for technology development using Proteomics systems for Biomarker Discovery and Multiplex Immunoassays. Previously, at Beckman Coulter, he was also involved in technology development using Genomic System for multiplex gene expression, genetic analysis (STR, SNPs and AFLP etc.) and sequencing.

    Abstract
    DATE:  December 10, 2019
    TIME:   7:00am PST, 10:00am EST
     
     
    Rapid growth in the cell and gene therapy industry has generated an urgent need for robust analytics around characterization of both vectors and nucleic acid biotherapeutics such as oligonucleotides, plasmid DNA, small RNA, mRNA and double stranded DNA.
     
    Adeno-associated virus (AAV) is one of the most widely used gene delivery vehicles for gene therapy because of its non-pathogenicity, low immunogenicity and different tropism to multiple cell types. Although there are efforts to develop methods for characterization of AAV, there are still limitations and drawbacks to some of these analyses. In addition, the different workflows are done on different platforms. Here we propose multiple key analytical workflows that can be performed on a single Capillary electrophoresis (CE) based platform for extensive characterization of AAV. Some of these methodologies include AAV capsid purity analysis (VP1, VP2 and VP3) using both UV and laser-induced fluorescence (LIF) detection.
     
    In addition, a suite of analytical methods recently developed and optimized for characterization of the nucleic acid biotherapeutics and detection of impurities. Presented here is a ready-to-use protocol for not only in-depth characterization of various nucleic acid but also analysis of impurities in these biotherapeutics. Furthermore, all of these methods for vector and nucleic acid analysis are developed on a GMP compliant instrument with associated reagent kits and consumables commercially available; thus, making it a seamless process to analyze these newer modalities.
     
    Learning Objectives:
    • Discover Solutions for Sizing, Purity, and Sequence Analysis of Your Cell and Gene Therapeutics
    • Easily analyze a wide range of nucleic acids, viral capsids, & proteins all on one platform
    • Increase data confidence with robust solutions utilizing industry and regulatory proven technology 
     
     
    Webinars will be available for unlimited on-demand viewing after live event.
     
    LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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    Proven Answers for Protein, Vaccine and Gene Therapy Analysis

    Sponsored by: SCIEX
    C.E. Credits: P.A.C.E. CE Florida CE

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