FEB 07, 2018 12:00 PM PST

Refinement of a Model of Repeated Cerebrospinal Fluid Collection in Conscious Rats

C.E. CREDITS: RACE
Speakers
  • Clinical Veterinarian, Comparative Medicine, Basel, Roche Pharma Research and Early Development, Roche Innovation Center Basel
    Biography
      Eva Maria Amen, Dr. med vet, has been working as Clinical veterinarian at F. Hoffmann-La Roche Ltd, Department of Comparative Medicine, in Basel, Switzerland since September 2011. She received her veterinary degree at the University of Veterinary Medicine in Hannover, Germany, followed by an Internship at the Small Animal Clinic and a doctoral thesis at the Department of Pathology of the same university. Since 2009 she has been working in the field of laboratory animal medicine, first at the Bayer Pharmaceutical research Center in Wuppertal, Germany, and thereafter at her current employer in Switzerland, and she is enrolled as ECLAM resident since July 2013. In her role as Clinical Veterinarian she has been supporting and advising other research functions on research with animals and providing surgical support in rat and large animal models. Born in Germany and raised in Ecuador, Eva Maria truly enjoys working in an international environment and meeting and communicating with people from all over the world.

    Abstract:

    Introduction:
    The cannulation of the cisterna magna in the rat is a valuable model for in-vivo sampling of cerebrospinal fluid for studying delivery of new drugs into the central nervous system or disease models. It offers the advantages of repeated sampling without anesthesia-induced bias and using animals as their own controls.

    Materials and Methods:
    An established model was retrospectively reviewed for outcome and it was hypothesized that by refining the method i.e. by 1) implementing pathophysiological based anesthesia & analgesia, 2) using state of the art peri-operative monitoring and supportive care, 3) increasing stability of the cement-cannula assembly, and 4) selecting a more adapted animal strain, the outcome of the model – quantified by peri-operative mortality, survival time and stability of the implant- could be improved and enhance animal welfare.

    Results:
    After refinement of the technique, perioperative mortality significantly dropped (7 animals out of 73 compared to 4 out of 322; p=0.001), survival time significantly increased (36±14 compared to 28±18 days; p<0.001), as well as the stability of the cement-cannula-assembly (47±8 days of adhesion compared to 33±15 days and 34±13 days with two other cement types; p<0.001).

    Discussion and Conclusions:
    Overall, the concept of the 3 Rs by Russel and Burch was successfully addressed and animal welfare improved by: 1) reducing the total number of animals needed thanks to lower mortality or euthanasia due to technical failure and increased usage time frame of the individual rats; 2) improving the scientific quality of the model.


    Show Resources
    You May Also Like
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    NOV 18, 2019 07:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    NOV 18, 2019 07:00 AM PST
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Loading Comments...
    Show Resources