FEB 07, 2018 12:00 PM PST

Refinement of a Model of Repeated Cerebrospinal Fluid Collection in Conscious Rats

Speaker
  • Clinical Veterinarian, Comparative Medicine, Basel, Roche Pharma Research and Early Development, Roche Innovation Center Basel
    Biography
      Eva Maria Amen, Dr. med vet, has been working as Clinical veterinarian at F. Hoffmann-La Roche Ltd, Department of Comparative Medicine, in Basel, Switzerland since September 2011. She received her veterinary degree at the University of Veterinary Medicine in Hannover, Germany, followed by an Internship at the Small Animal Clinic and a doctoral thesis at the Department of Pathology of the same university. Since 2009 she has been working in the field of laboratory animal medicine, first at the Bayer Pharmaceutical research Center in Wuppertal, Germany, and thereafter at her current employer in Switzerland, and she is enrolled as ECLAM resident since July 2013. In her role as Clinical Veterinarian she has been supporting and advising other research functions on research with animals and providing surgical support in rat and large animal models. Born in Germany and raised in Ecuador, Eva Maria truly enjoys working in an international environment and meeting and communicating with people from all over the world.

    Abstract

    Introduction:
    The cannulation of the cisterna magna in the rat is a valuable model for in-vivo sampling of cerebrospinal fluid for studying delivery of new drugs into the central nervous system or disease models. It offers the advantages of repeated sampling without anesthesia-induced bias and using animals as their own controls.

    Materials and Methods:
    An established model was retrospectively reviewed for outcome and it was hypothesized that by refining the method i.e. by 1) implementing pathophysiological based anesthesia & analgesia, 2) using state of the art peri-operative monitoring and supportive care, 3) increasing stability of the cement-cannula assembly, and 4) selecting a more adapted animal strain, the outcome of the model – quantified by peri-operative mortality, survival time and stability of the implant- could be improved and enhance animal welfare.

    Results:
    After refinement of the technique, perioperative mortality significantly dropped (7 animals out of 73 compared to 4 out of 322; p=0.001), survival time significantly increased (36±14 compared to 28±18 days; p<0.001), as well as the stability of the cement-cannula-assembly (47±8 days of adhesion compared to 33±15 days and 34±13 days with two other cement types; p<0.001).

    Discussion and Conclusions:
    Overall, the concept of the 3 Rs by Russel and Burch was successfully addressed and animal welfare improved by: 1) reducing the total number of animals needed thanks to lower mortality or euthanasia due to technical failure and increased usage time frame of the individual rats; 2) improving the scientific quality of the model.


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    APR 01, 2021 8:00 AM PDT
    C.E. CREDITS
    APR 01, 2021 8:00 AM PDT
    Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
    FEB 07, 2018 12:00 PM PST

    Refinement of a Model of Repeated Cerebrospinal Fluid Collection in Conscious Rats


    Specialty

    Animal Models

    Animal Research

    Animal Sciences

    Research

    Animals

    Animal Behavior

    Veterinary Sciences

    Brain

    Molecular Diagnostics

    Molecular Biology

    Research And Development

    Neuroscience

    Lab Animals

    Neurobiology

    Therapy

    Geography

    Europe81%

    North America13%

    Asia6%

    Registration Source

    Website Visitors100%

    Job Title

    Medical Doctor/Specialist25%

    Executive17%

    Research Scientist17%

    Scientist17%

    Microbiologist8%

    Veterinarian8%

    Post Doc8%

    Organization

    Academic Institution31%

    Pharmaceutical Company13%

    Hospital13%

    Clinical Laboratory6%

    Medical Center6%

    Contract Research Organization (Cro)6%

    Research Institute6%

    Medical School6%

    Government/public6%

    Government6%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more