After new users become familiar with flow cytometry instrumentation and learn how to set up the instrument and acquire data, the next topic to master is panel design. Optimizing a multicolor immunophenotyping panel is often feared as an iterative, time-consuming and costly process with a lot of uncertainty about the true performance characteristics of the final design. Simultaneous optimization of multiple variables is required, empirically trading off sensitivity due to marker co-expression and inherent instrument limitations.
In this webinar, Matt Goff and Michael Kapinsky from our research flow cytometry platform will discuss panel design from both the assay development and the instrumentation development perspective. In the first part of this webinar we describe a practical stepwise method that organizes panel design principles in an easily understand diagram. In the second part of the webinar we will use these principles to give an understanding of how new flow cytometry technology facilitates immunophenotyping experiments. Using flow cytometry data, we’ll compare the experimental outcomes of panels with the results expected according to panel design principles.