NOV 16, 2017 11:00 AM PST

WEBINAR: Get Results Faster: Translating your Assay to High Content Analysis

Sponsored by: Cytiva
Speaker
  • HCA Field Applications Scientist, GE Healthcare
    Biography
      Dr. Maggie Vantangoli Policelli received her B.S. from Bridgewater State College, and Ph.D. from Brown University, where her research centered on the development of novel three-dimensional platforms for toxicity testing. This research integrated toxicological, morphological and molecular endpoints using high-content imaging-based approaches.

    Abstract

    DATE: November 16, 2017
    TIME: 11:00am PST, 2:00pm EST

    High Content Analysis (HCA) is a technique utilizing automated microscopy to obtain phenotypic and morphological information. This data rich approach allows you to extract 70+ measurements from every cell in every image. Did you know that HCA is not just for screening and drug discovery? This powerful technique can be used to optimize experimental conditions, quantify large numbers of cells, increase replicates and is applicable to many routine experiments. Attend this webinar to understand how to confidently translate your current assay to HCA. You will learn how to optimize both your biology and acquisition in order to quickly make new biological insights using High Content Analysis.

    Key Learning Points:

    • Which plate to choose for your assay to get the best image quality and quantification
    • How to determine the correct seeding density and labeling conditions to accurately segment your images
    • Which objective to pick to balance the number of cells and field of view size
    • What type of autofocus will be best for your experiment to maintain focus during acquisition
    • What binning is and why you should consider it to manage the volume of data generated
    • How to optimize your acquisition settings to get the best signal to background

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