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MAY 10, 2017 9:00 AM PDT

A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System

Speaker
  • Scientist, high-throughput transient transfectio, Pfizer, Inc.
    Biography
      2000-2001 Genzyme Biosurgery, GMP Culture of autologous chondrocytes for knee replacement surgery
      2001-2002 Harvard School of Public Health, Responsible for culturing of Human Airway Smooth Muscle cells for asthma research
      2002-2007 ImmunoGen, Responsible for the stable cell line development of DG44CHO cells secreting monoclonal antibodies
      2007-2010 Organon/Schering-Plough/Merck, Responsible for the stable and large scale transient development of DG44CHO and Hek293F cells to produce monoclonal antibodies
      2010-Present Pfizer, Responsible for large scale and small scale transient expression of proteins in various formats using the Expi293F transient transfection platform. Currently working in the High Throughput Transient transfection group

    Abstract

    We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stock with an automated DNA purification system, followed by semi-automated transfection/feed/clarification/filtration and a fully-automated purification and buffer exchange, we deliver high quality-protein supporting dozens of projects with an eight-day turnaround time.


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    MAY 10, 2017 9:00 AM PDT

    A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System


    Specialty

    Biotechnology

    Cancer Research

    Antibodies

    Cancer Therapeutics

    Cell Biology

    Drug Discovery

    Biochemistry

    Pcr/rt-Pcr/real-Time Pcr

    Gene Expression

    Bioinformatics

    Clinical Research

    Gene Sequencing

    Molecular Biology

    Research

    Crispr-Cas9

    Geography

    North America55%

    Asia24%

    South America17%

    Europe2%

    Africa2%

    Registration Source

    Website Visitors100%

    Job Title

    Student24%

    Scientist21%

    Research Scientist18%

    Executive15%

    Medical Laboratory Technician6%

    Marketing/Sales3%

    Post Doc3%

    Lab Management3%

    Medical Doctor/Specialist3%

    Facility/Department Manager3%

    Educator/Faculty3%

    Organization

    Biotech Company19%

    Academic Institution14%

    Life Science Company10%

    Ambulatory Care5%

    Private Practice2%

    Non-Profit Organization2%

    Hospital2%

    Manufacturer - Other2%

    Government2%


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