MAY 10, 2017 09:00 AM PDT

A Semi-Automated High-Throughput Transient Expression and Purification Platform using the Expi293 Expression System

Speakers
  • Scientist, high-throughput transient transfectio, Pfizer, Inc.
    Biography
      2000-2001 Genzyme Biosurgery, GMP Culture of autologous chondrocytes for knee replacement surgery
      2001-2002 Harvard School of Public Health, Responsible for culturing of Human Airway Smooth Muscle cells for asthma research
      2002-2007 ImmunoGen, Responsible for the stable cell line development of DG44CHO cells secreting monoclonal antibodies
      2007-2010 Organon/Schering-Plough/Merck, Responsible for the stable and large scale transient development of DG44CHO and Hek293F cells to produce monoclonal antibodies
      2010-Present Pfizer, Responsible for large scale and small scale transient expression of proteins in various formats using the Expi293F transient transfection platform. Currently working in the High Throughput Transient transfection group

    Abstract:

    We have developed a semi-automated, high-throughput transient expression and purification system that yields milligram quantities of hundreds of proteins weekly. Starting from a glycerol stock with an automated DNA purification system, followed by semi-automated transfection/feed/clarification/filtration and a fully-automated purification and buffer exchange, we deliver high quality-protein supporting dozens of projects with an eight-day turnaround time.


    Show Resources
    You May Also Like
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 02, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 02, 2018 08:00 AM PDT
    Immunohistochemistry protocols, which utilize antibodies to visualize proteins in tissue sections, have many steps that need optimized to prevent non-specific background effects, artifacts, o...
    JUN 29, 2018 09:00 AM PDT
    C.E. CREDITS
    JUN 29, 2018 09:00 AM PDT
    DATE: June 29, 2018TIME: 09:00AM PDT, 12:00PM EDT There is significant epidemiological evidence to suggest that the consumption of a high-broccoli diet is associated with a r...
    MAY 29, 2018 09:00 AM PDT
    C.E. CREDITS
    MAY 29, 2018 09:00 AM PDT
    Recent advances in immuno-oncology research has proven the critical role of immune cells in cancer therapy. Deep understanding of the interaction and phenotype of immune and tumor cells withi...
    Loading Comments...