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MAY 16, 2018 11:00 PM HKT

A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)

Speaker

Abstract

The baculovirus expression vector system (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically-defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant lot-to-lot variability in terms of both cell growth and protein expression. Here, we present the development of a novel Sf9-based baculovirus expression system based on a high-density, chemically-defined culture medium, a high-expressing Sf9 cell line and expression enhancers that allow for the consistent production of recombinant proteins with two-fold or greater improvements in protein titers compared to traditional BEVS workflows.

摘要

杆状病毒表达载体系统(BEVS)是重组蛋白生产的主要平台之一。与哺乳动物表达系统早已转换为无血清,化学成分限定的培养基不同,昆虫表达系统的创新相对较少。昆虫细胞继续依赖成分不限定的含酵母提取物的培养基,而这些培养基可以显示出明显的细胞生长和蛋白质表达的批次差异。在这里,我们介绍一种新型的Sf9细胞杆状病毒表达系统的开发。此系统包括支持高细胞密度、化学成分限定的培养基,高表达Sf9细胞系,以及优化的培养辅料。此表达系统提供一致的重组蛋白生产,且比传统的BEVS提高了两倍或更多的蛋白滴度。


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MAY 16, 2018 11:00 PM HKT

A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)



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