MAY 17, 2018 02:00 PM HKT

A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)

  • Field Application Specialist, Thermo Fisher Scientific
      Yang Nan is a technical support for the expression of instantaneous protein in the European region of Thermo Fisher Technology. She received a bachelor's degree in life Science from Nanyang Technological University in Singapore in 2006, followed by a PhD in biofilm research at the National Institute of Applied Sciences in Lyon, France, in 2011. 楊楠是賽默飛世爾科技在歐洲地區表達瞬時蛋白質的技術支持。 她於2006年獲得新加坡南洋理工大學生命科學學士學位,隨後於2011年在法國里昂國家應用科學研究所獲得生物膜研究博士學位。


    The baculovirus expression vector system (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically-defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant lot-to-lot variability in terms of both cell growth and protein expression. Here, we present the development of a novel Sf9-based baculovirus expression system based on a high-density, chemically-defined culture medium, a high-expressing Sf9 cell line and expression enhancers that allow for the consistent production of recombinant proteins with two-fold or greater improvements in protein titers compared to traditional BEVS workflows.



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