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MAY 16, 2018 11:00 PM HKT

A chemically defined baculovirus-based expression system for enhanced protein production in Sf9 cells (presented in Chinese)

Speaker
  • Field Application Specialist, Thermo Fisher Scientific
    Biography
      Yang Nan is a technical support for the expression of instantaneous protein in the European region of Thermo Fisher Technology. She received a bachelor's degree in life Science from Nanyang Technological University in Singapore in 2006, followed by a PhD in biofilm research at the National Institute of Applied Sciences in Lyon, France, in 2011. 楊楠是賽默飛世爾科技在歐洲地區表達瞬時蛋白質的技術支持。 她於2006年獲得新加坡南洋理工大學生命科學學士學位,隨後於2011年在法國里昂國家應用科學研究所獲得生物膜研究博士學位。

    Abstract

    The baculovirus expression vector system (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically-defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant lot-to-lot variability in terms of both cell growth and protein expression. Here, we present the development of a novel Sf9-based baculovirus expression system based on a high-density, chemically-defined culture medium, a high-expressing Sf9 cell line and expression enhancers that allow for the consistent production of recombinant proteins with two-fold or greater improvements in protein titers compared to traditional BEVS workflows.

    摘要

    杆状病毒表达载体系统(BEVS)是重组蛋白生产的主要平台之一。与哺乳动物表达系统早已转换为无血清,化学成分限定的培养基不同,昆虫表达系统的创新相对较少。昆虫细胞继续依赖成分不限定的含酵母提取物的培养基,而这些培养基可以显示出明显的细胞生长和蛋白质表达的批次差异。在这里,我们介绍一种新型的Sf9细胞杆状病毒表达系统的开发。此系统包括支持高细胞密度、化学成分限定的培养基,高表达Sf9细胞系,以及优化的培养辅料。此表达系统提供一致的重组蛋白生产,且比传统的BEVS提高了两倍或更多的蛋白滴度。


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