JUL 13, 2016 09:00 AM PDT

Solana GAS Molecular Assay...No Culture Confirmation Required!

SPONSORED BY: Quidel, Quidel

Abstract:

The Solana GAS Assay is a rapid in vitro diagnostic test for the qualitative detection of Group A β-hemolytic Streptococcus (Streptococcus pyogenes) nucleic acids isolated from throat swab specimens obtained from patients with signs and symptoms of pharyngitis, such as sore throat.

The Solana GAS Assay, when performed on the Solana instrument, allows for the rapid, accurate detection of Group A Strep without the need for culture confirmation.

The assay utilizes helicase-dependent amplification (HDA) of the DNase B (sdaB) sequence and fluorescent probe-based detection in the Solana instrument to determine assay results.

A throat swab sample or an aliquot of liquid transport media is lysed by simple heat treatment, diluted, and added to a Reaction Tube containing the lyophilized HDA reagents including primers specific for the amplification of the DNAase B (sdaB) sequence as well as sequence specific probes. Competitive process control (PRC) is included in the Lysis Tube to monitor sample processing, inhibitory substances in clinical samples, reagent failure or instrument failure.

Results are displayed on the touchscreen, can be saved to the instrument, printed, and are capable of being sent to the LIS and exported through one of Solana’s six USB ports. Solana GAS is supported by the power of Virena
 


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