JUL 25, 2017 9:00 AM PDT

WEBINAR: Functional Genomics and Drug Metabolism

Sponsored by: Thermo Fisher Scientific



DATE: July 25, 2017
TIME: 9:00am PT, Noon ET

Thermo Fisher Scientific is proud to present the SyncD3 webinar series.  As a thought-leader in science our first commitment is to you the scientist and partnering to drive the conversation on current practices, standards and the development of innovation. 

Join us for our continuation of the SyncD3 webinar series and virtual event, where we will discuss cutting-edge tools and topics in the field(s) of Drug Discovery & Development such as:

  • HCS/Phenotypic Assays
  • Functional Genomics & Drug Metabolism
  • 3D models/Organoids
  • Diseased models
  • Stem Cells

We will discuss where these areas intersect and impact drug discovery and development and the future of the industry during a live panel discussion with our presenters and you the audience.  Learn from real field scientists and researchers who are working in the ADME/Tox and Drug Discovery fields and bring your questions for the open forum Q&A session after our presentations. 

If you cannot make the live presentation you are always capable of accessing the recorded information, presentations and Q&A any time after the event by registering here.

Unraveling biology and identifying targets with functional genomics approaches supported by LentiArray CRISPR library screens

Identifying and validating targets that underlie disease mechanisms and can be addressed to provide efficacious therapies remains a significant challenge in the drug discovery and development process.  Mechanisms of RNAi have provided the use of siRNA and shRNA to knock-down RNA and suppress gene function.  However, depending on the nature of the targets, cells, biology and end-point assays, these approaches may suffer variously from their transient nature, design complexity, incomplete knock-down or off-target effects.  The use of CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease and guide RNA (gRNA) provides a strong alternative that can produce transient or long-lasting impact, straightforward design, knock-out of genes and increased specificity.  A number of laboratories have already published reports demonstrating how pools of gRNA can be delivered to cells and “hits” can be established through enrichment or depletion of cells following a “survival” assay and identified by sequencing the introduced gRNAs in the remaining cell population.  Here we demonstrate a knock-out screening approach that utilizes the Invitrogen™ LentiArray™ CRISPR library to interrogate the impact of individual gene knock-outs on the NFκB pathway as measured by a functional cell-based assay.  We describe the library design concepts, the assay development, initial screening results and validation of specific identified hits. We elucidate the key factors in developing a robust assay including both transduction and assay optimization to achieve the highest levels of transduction efficiency and assay window and provide data from initial screens using the Invitrogen™ LentiArray™ CRISPR kinome library.  We expect these approaches to be scaleable to the entire human genome and portable to multiple cell types and end-point assays including both high-throughput plate-based assays and high-content imaging based assays.

Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
JUN 09, 2021 7:00 AM PDT
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
JUL 15, 2021 9:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
MAR 16, 2021 10:00 AM PDT
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
APR 21, 2021 5:00 PM CEST
APR 21, 2021 5:00 PM CEST
Date: April 21, 2021 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Spatial Answers Trilogy - Spatial Answers in Immunology Immunology Researchers share their Spatial Discoveries in SARS-C...
APR 01, 2021 8:00 AM PDT
APR 01, 2021 8:00 AM PDT
Date: April 01, 2021 Time: 8:00am (PST), 11:00am (EST) Generating therapeutic antibodies is far more challenging than obtaining antibodies that merely recognize their targets. Engineering po...
JUL 25, 2017 9:00 AM PDT

WEBINAR: Functional Genomics and Drug Metabolism

Sponsored by: Thermo Fisher Scientific


Molecular Biology

Cancer Research

Cell Biology




Molecular Diagnostics


Cell Culture

Clinical Diagnostics

Cancer Diagnostics


Cancer Therapeutics

Laboratory Testing



North America59%



South America3%


Registration Source

Website Visitors100%

Job Title

Research Scientist15%





Medical Laboratory Technician8%

Post Doc8%

Facility/Department Manager6%

Lab Management6%

Clinical Laboratory Scientist4%

Medical Doctor/Specialist4%






Academic Institution11%

Clinical Laboratory8%

Research Institute6%

Biotech Company5%

Life Science Company3%



Pharmaceutical Company3%

Medical School2%

Ambulatory Care2%


Non-Profit Organization1%

Contract Research Organization (Cro)1%

Medical Center1%


Show Resources
Loading Comments...
Show Resources