FEB 05, 2014 11:00 AM PST

The Future of Mouse Embryo Transfer: Achieving the 3Rs with the NSET Device

C.E. Credits: CE
Speaker
  • Senior Research Scientist, NSET testing and development Chair, ParaTechs Corp IACUC, ParaTechs Corporation, USA
    BIOGRAPHY

Abstract

Non-surgical transfer techniques for mouse embryos and sperm provide animal welfare benefits for assisted reproduction of mice. While surgical embryo transfer (ET) is an effective method for transfer of embryos into the uterine horn of mice, surgery is expensive, time consuming, and requires technical expertise. Surgery is also a stressful procedure for the mouse, which has to be anesthetized and treated with an analgesic. We have developed a simple, brief procedure for ET using a non-surgical embryo transfer (NSET) device. Once embryos are loaded into the NSET device, the tapered NSET catheter passes through the vagina and traverses the cervix to deposit the embryos into the uterine horn of a recipient mouse. Since the NSET procedure does not require sedation, opening of the inner body cavity, or use of an analgesic, this procedure is less stressful for the mouse than surgery. In proof, our results show that when pseudopregnant mice are monitored by electrocardiography, the non-surgical procedure does not affect heart rate. The NSET procedure also has no effect on the level of the stress biomarker fecal corticosterone measured by ELISA analysis. However, surgery or anesthesia does lower heart rate and increases levels of fecal corticosterone. Additional studies show that the NSET procedure is effective for transfer of mouse embryos for the purposes of rederivation, cryorecovery, and transgenic research. Embryos can be successfully transferred with the NSET technique after cryopreservation, in vitro fertilization (IVF), or embryonic stem (ES) cell injection as an alternative to traditional surgical embryo transfer. The NSET device has also successfully transferred mouse sperm for artificial insemination. Therefore, the NSET technique is an example of a 3Rs refinement, an alternative to a surgical procedure, which will lead the way for improved assisted reproductive techniques in mice.


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FEB 05, 2014 11:00 AM PST

The Future of Mouse Embryo Transfer: Achieving the 3Rs with the NSET Device

C.E. Credits: CE

Specialty

Dna

Cell Culture

Lab Animals

Biotechnology

Animal Models

Gene Expression

Animal Sciences

Research

Cancer Research

Animals

Neurosurgery

Dna Sequencing

Personalized Medicine

Drug Development

Immunity

Geography

Europe67%

North America33%

Registration Source

Website Visitors100%

Job Title

Scientist33%

Student22%

Research Scientist22%

Educator/Faculty11%

Facility/Department Manager11%

Organization

Academic Institution56%

Pharmaceutical Company33%

Research Institute11%


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