SEP 08, 2016 7:30 AM PDT

The Molecular Pathology Perspective of the CDC's Infectious Diseases Pathology Branch

Speaker
  • Microbiologist, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
    BIOGRAPHY

Abstract

The Infectious Diseases Pathology Branch of the Centers for Disease Control and Prevention (CDC) routinely receives autopsy and biopsy tissues for diagnostic evaluation. These tissues are typically formalin-fixed, paraffin-embedded (FFPE) in nature. Initially, histological stains are employed to determine which tissues are affected and the nature of the infectious process. Along with any provided clinical information, this information is then used by the pathologist to request testing for specific pathogens, either by use of immunohistochemical or molecular techniques. As one of the molecular pathologists, my primary tools are PCR and RT-PCR assays specific to bacterial or viral agents. After agarose gel analysis of the amplicons, followed by band excision and purification, Sanger sequencing can be performed using the GenomeLab GeXP™ Genetic Analysis System. Sequences are then trimmed, contigs are created, and consensus sequences are analyzed using the Basic Local Alignment Search Tool (BLAST). Depending on the level of identity reported by the BLAST, specific diagnoses can then be made, providing clinicians, patients, and/or patient’s families with valuable insight. While the process sounds simple and straightforward, there are actually many difficulties that arise when working with FFPE tissues. However, FFPE tissues provide a convenient means to examine tissues for infectious organisms from a variety of geographical locations using multiple modalities with no biosafety risk.
 


Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
JUN 21, 2022 6:00 AM PDT
JUN 21, 2022 6:00 AM PDT
Date: June 21, 2022 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) The global understanding and practice of medicine is currently undergoing a revolutionary change. This shift to precision...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
OCT 05, 2022 9:00 AM PDT
C.E. CREDITS
OCT 05, 2022 9:00 AM PDT
Date: October 05, 2022 Time: 9:00am (PST), 12:00pm (EST), 6:00pm (CEST) Candida auris is a multi-drug resistant yeast that continues to be a global threat for infection and transmission in h...
SEP 08, 2016 7:30 AM PDT

The Molecular Pathology Perspective of the CDC's Infectious Diseases Pathology Branch



Show Resources
Loading Comments...
Show Resources