MAY 10, 2016 7:00 AM PDT

The Tip of the Iceberg - How Pipette Tips Influence Assay Results

Sponsored by: Eppendorf, Eppendorf
Speakers
  • Field Application Specialist Liquid Handling, Eppendorf AG
    Biography
      Ulrike Gast specializes in applications for liquid handling systems and teaching customers their correct use. She has supported several Eppendorf pipette calibration services world-wide in their accreditation process. Within the frame of the development of a new guideline (DKD R 8-1), she was member of the responsive international technical committee of DKD, an official body for calibration solutions. The guideline defines technical requirements for the calibration of liquid handling systems and the set-up of measurement uncertainty budget for calibration labs.

      Ulrike Gast holds a Ph.D. in biology from the University of Tübingen based on a broad spectrum of methods. She has a great deal of practical experience in both the correct use of liquid handling systems and their performance testing.

    Abstract:
    DATE: May 10th, 2016
    TIME:  7am Pacific time, 10am Eastern time, 4pm Central European Summer time


    When was the last time you were upset about non-reproducible results of your experiment? Even though you checked all variables including the pipetting system used in your experiment, you were unable to find the cause.
     
    In many cases the assumption is made that the pipetting system, consisting of pipette and pipette tips, cannot be a source of error as long as the tip fits the pipette. However, even if this is the case, this does not speak to the precision and accuracy of the pipetting result. Therefore the international standard ISO 8655:2002 recommends the use of pipettes and tips of the same manufacturer. With good reason!
     
    In this webinar we will show the results of our comprehensive study about tip quality in system with the pipette. At the end of this webinar you will have an overview of several influencing factors impairing your pipetting results when using pipettes and tips. We found that in the same way that only the tip of an iceberg can be seen above water level, a number of other problems with pipette tips rather stay unknown.
     

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