MENU
JUN 20, 2019 12:00 PM PDT

Tissue and cfDNA Rare Alleles: How Low Can We Go?

C.E. Credits: P.A.C.E. CE Florida CE
Speaker
  • Director Scientific Collaboration , Department of Genetics, Washington University School of Medicine
    Biography
      Seth Crosby is the Director of Science Collaboration in the Department of Genetics at Washington University. Crosby works with groups within and outside the University to leverage technology to achieve research and clinical goals. He has been at Washington University for 16 years. During that time, he directed the Department of Genetics Microarray Core, which is now part of GTAC and then GTAC, itself, during its first two years. Prior to that he was the Director of Translational Research at the Genome Center (now the McDonnell Genome Institute). Prior to his tenure at Washington University, Dr. Crosby worked in the field of genomics and drug discovery at Abbott Laboratories, Pfizer and Pharmacia.

    Abstract

    This talk will review successful efforts at Washington University to employ novel reagents and informatics to the problem of rare allele detection. I will discuss a published bakeoff of in silico variant callers. I will describe rare variant detection in our lab using a novel “unlockable” blocked PCR primer developed at IDT. Finally I will describe a new method, developed at Washington University to leverage UMI to push detection down to 1 in 10,000.

    Learning Objectives: 

    1. Will discuss bench versus bioinformatic approaches to sensitivity to rare alleles both in malignant tissue and in a so-called ”liquid biopsy".
    2. Will discuss the method that proports maximum sensitivity (0.01)


    Show Resources
    You May Also Like
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    MAY 08, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    FEB 19, 2020 11:00 AM PST
    C.E. CREDITS
    FEB 19, 2020 11:00 AM PST
    DATE: February 19, 2020TIME: 11:00am PST, 2:00pm EST...
    NOV 18, 2019 7:00 AM PST
    C.E. CREDITS
    NOV 18, 2019 7:00 AM PST
    DATE: November 18, 2019TIME: 7:00am PST, 11:00am EST, 4:00pm CEWT How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop th...
    Loading Comments...
    Show Resources