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JUN 20, 2019 12:00 PM PDT

Tissue and cfDNA Rare Alleles: How Low Can We Go?

Speaker
  • Director Scientific Collaboration , Department of Genetics, Washington University School of Medicine
    BIOGRAPHY

Abstract

This talk will review successful efforts at Washington University to employ novel reagents and informatics to the problem of rare allele detection. I will discuss a published bakeoff of in silico variant callers. I will describe rare variant detection in our lab using a novel “unlockable” blocked PCR primer developed at IDT. Finally I will describe a new method, developed at Washington University to leverage UMI to push detection down to 1 in 10,000.

Learning Objectives: 

1. Will discuss bench versus bioinformatic approaches to sensitivity to rare alleles both in malignant tissue and in a so-called ”liquid biopsy".
2. Will discuss the method that proports maximum sensitivity (0.01)


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JUN 20, 2019 12:00 PM PDT

Tissue and cfDNA Rare Alleles: How Low Can We Go?



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