MENU

In vitro research method for screening inhibitors of protein translation

Speaker
  • Senior R & D Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
    Biography
      Krishna's PhD and postdoctural work focused on understanding control mechanisms regulating the initiation of protein translation in eukaryotes. Following his academic training, he joined Thermo Fisher Scientific and has led multiple projects including the development of the HeLa and CHO lysate based cell-free translation systems, also known asThermo Scientific1-Step In VitroTranslation (IVT) Systems, which contain all the necessary reagents to express proteins of interest in as little as one hour.

    Abstract

    In vitro translation (IVT), or cell-free expression, offers a unique and powerful research tool to screen for translational inhibitors that regulate both cellular and viral protein expression. Here we discuss a simple, quick method to identify inhibitors of both cap-dependent and cap-independent protein translation, using the Thermo Scientific1-Step Human Coupled IVT Kit. Rapid assay readout (6090 min), amenability for miniaturization, and insensitivity to compound toxicity make in vitro translation an attractive alternative to cell-based screens for high-throughput screening (HTS) of novel inhibitors of proteinsynthesis


    Show Resources
    You May Also Like
    APR 07, 2020 8:00 AM PDT
    C.E. CREDITS
    APR 07, 2020 8:00 AM PDT
    DATE: April 7, 2020 TIME: 8:00am PT, 11:00am ET This webinar sets out to establish why quality control is key to robust, reliable, reproducible science. We will look at best practice criteri...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    AUG 27, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    AUG 27, 2020 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: August 27, 2020 TIME: 7:00am PT Novel anti-viral medications, including biologics and small molecule inhibitors, as well as serologic diagnostic tool sets are in urgent demand to fight...
    MAY 08, 2020 10:00 AM PDT
    C.E. CREDITS
    MAY 08, 2020 10:00 AM PDT
    DATE: May 8, 2020 TIME: 10:00am PT, 11:00am MT, 1:00pm ET The application of next generation sequencing to interrogate immune repertoires and methods in which these highly complex dataset...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    MAY 12, 2020 9:00 AM PDT
    C.E. CREDITS
    MAY 12, 2020 9:00 AM PDT
    NEW DATE: Date: May 12, 2020 Time: 9:00am PDT, 12:00pm EDT...
    Loading Comments...
    Show Resources