JAN 19, 2017 8:00 AM PST

Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR

Sponsored by: ArcherDX, ArcherDX
Speakers
  • Chief Scientific Officer and General Manager at ArcherDX
    Biography
      Originally from Baltimore, MD, Josh Stahl studied biochemistry and molecular biology at West Virginia University. He went to graduate school at the University of Colorado, Boulder, working on protein-protein interactions in morphine analgesia.

      Josh joined ArcherDX in 2013, and has overseen biochemistry, molecular biology, and software development since that time. Now general manager and chief scientific office, Josh continues to lead Archer R&D, product development and operations teams in developing new applications and Archer technology
    • Product Manager at ArcherDX
      Biography
        Jonathan Doose completed his post-graduate studies at the University of California, Riverside where he investigated the molecular mechanism of CNS immune privilege in microglia and potential new therapeutic targets in neurodegenerative autoimmune diseases. He joined ArcherDX in early 2016 and manages FusionPlex and VariantPlex assay development. Jonathan was essential in bringing the Archer Reveal ctDNA assay to market and continues to harness his scientific expertise to develop new applications for Archer technology.

      Abstract

      DATE: January 19th, 2017
      TIME: 8:00AM PT, 11:00AM ET


      Liquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational status. Cell free DNA (cfDNA) can be profiled from liquid biopsy samples, although the abundance is generally low, ranging from less than 1ng to 30ng cfDNA per mL blood in healthy individuals. Furthermore, only a small fraction of cfDNA originates from tumor cells as circulating tumor DNA (ctDNA), except in more advanced disease states. This ctDNA is typically highly fragmented (100-300bp), and thus NGS-based methods to detect oncogenic driver mutations from liquid biopsies must be sensitive enough to detect low-frequency mutations (allele fractions less than 2%) from low inputs (10-100ng) of highly fragmented material.
       
      Anchored Multiplex PCR (AMP™) is proprietary target enrichment chemistry for NGS that is uniquely suited for highly fragmented material such as FFPE-derived nucleic acids and liquid biopsy-derived ctDNA. In this webinar, Josh Stahl, Chief Scientific Officer and General Manager at ArcherDX, will describe AMP-based ctDNA library preparation method, highlighting the use and advantages of molecular barcoded adapters used to unambiguously remove PCR duplicates, correct for PCR or sequencer-derived sequence errors, and flag run-to-run contamination. Josh will further talk about how the Archer® Reveal ctDNA™ 28 kit combines robust variant detection and quantitative analysis with single-day library prep for powerful, accurate and fast mutation analysis from liquid biopsies.
       
      Learning objective 1: Understand both the value of mutation analysis from ctDNA in liquid biopsies and the challenges of analyzing ctDNA by next-generation sequencing.
       
      Learning objective 2: Learn how the Archer Reveal ctDNA 28 kit uses Anchored Multiplex PCR target enrichment chemistry to detect and characterize ctDNA.

       


      Show Resources
      You May Also Like
      DEC 02, 2020 8:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      DEC 02, 2020 8:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
      OCT 08, 2020 7:00 AM PDT
      C.E. CREDITS
      OCT 08, 2020 7:00 AM PDT
      DATE: October 8, 2020 TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST How often do you pipette in your cell culture lab every day? Usually, we do it so often that we tend stop thinking about ho...
      OCT 29, 2020 6:00 AM PDT
      C.E. CREDITS
      OCT 29, 2020 6:00 AM PDT
      Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
      NOV 16, 2020 8:00 AM PST
      C.E. CREDITS
      NOV 16, 2020 8:00 AM PST
      Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
      SEP 10, 2020 9:00 AM PDT
      C.E. CREDITS
      SEP 10, 2020 9:00 AM PDT
      Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
      DEC 15, 2020 10:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      C.E. CREDITS
      DEC 15, 2020 10:00 AM PST
      Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
      DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
      Loading Comments...
      Show Resources
      Attendees
      • See more