JAN 19, 2017 8:00 AM PST

Webinar: Detecting meaningful mutations from liquid biopsies by Anchored Multiplex PCR

Sponsored by: ArcherDX, ArcherDX
Speakers

Abstract

DATE: January 19th, 2017
TIME: 8:00AM PT, 11:00AM ET


Liquid biopsies have the potential to be a less invasive method than traditional biopsies to detect advanced solid tumor mutational status. Cell free DNA (cfDNA) can be profiled from liquid biopsy samples, although the abundance is generally low, ranging from less than 1ng to 30ng cfDNA per mL blood in healthy individuals. Furthermore, only a small fraction of cfDNA originates from tumor cells as circulating tumor DNA (ctDNA), except in more advanced disease states. This ctDNA is typically highly fragmented (100-300bp), and thus NGS-based methods to detect oncogenic driver mutations from liquid biopsies must be sensitive enough to detect low-frequency mutations (allele fractions less than 2%) from low inputs (10-100ng) of highly fragmented material.
 
Anchored Multiplex PCR (AMP™) is proprietary target enrichment chemistry for NGS that is uniquely suited for highly fragmented material such as FFPE-derived nucleic acids and liquid biopsy-derived ctDNA. In this webinar, Josh Stahl, Chief Scientific Officer and General Manager at ArcherDX, will describe AMP-based ctDNA library preparation method, highlighting the use and advantages of molecular barcoded adapters used to unambiguously remove PCR duplicates, correct for PCR or sequencer-derived sequence errors, and flag run-to-run contamination. Josh will further talk about how the Archer® Reveal ctDNA™ 28 kit combines robust variant detection and quantitative analysis with single-day library prep for powerful, accurate and fast mutation analysis from liquid biopsies.
 
Learning objective 1: Understand both the value of mutation analysis from ctDNA in liquid biopsies and the challenges of analyzing ctDNA by next-generation sequencing.
 
Learning objective 2: Learn how the Archer Reveal ctDNA 28 kit uses Anchored Multiplex PCR target enrichment chemistry to detect and characterize ctDNA.

 


Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
JUN 28, 2022 7:00 AM PDT
JUN 28, 2022 7:00 AM PDT
Date: June 28, 2022 Time: 3:00pm (BST), 4:00pm (CET), 9:00am (CST), 7am (PST) Light-sheet microscopy is an extremely versatile imaging technique with a vast range of implementations that are...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
SEP 13, 2022 8:00 AM PDT
SEP 13, 2022 8:00 AM PDT
Date: September 13, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Preparedness against pandemic diseases demands rapid-response vaccine technology and ready-to-use analytical methods...
AUG 16, 2022 11:00 AM PDT
AUG 16, 2022 11:00 AM PDT
Date: August 16, 2022 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEST) Understanding genomic variation in the context of cancer is paramount to identifying disease drivers and informing pers...
LIVE NOW!
LIVE NOW!
Date: September 29, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Adeno-associated virus (AAV) is one of the most widely used delivery vehicles in gene therapy. To ensure the safety...
Loading Comments...
Show Resources