NOV 30, 2016 09:00 AM PST

Best practices in optimizing sample processing for improved flow cytometry

Speakers
  • Flow Cytometry Core Specialist, Keenan Research Centre for Biomedical Science, St. Michael's Research Hospital
    Biography
      I have been working in cell biology for 15 years, primarily working on analyzing the role of platelets in immunological and cardiovascular diseases. Most of my scientific career has been spent in laboratories in St Michael's Hospital, as well as McMaster University and the Australian Centre for Blood Diseases. I am currently the co-President of the Canadian Microscopy and Cytometry Association and have been involved with them since 2012.

      For the last 6 years I have run a flow cytometry and cell sorting core facility serving ~45 laboratories and 500 scientists in the Keenan Research Centre for Biomedical Science (KRCBS), St. Michael's Hospital. The core facility focuses on teaching individuals to do proper flow cytometry and consulting with scientists on their research programs rather than simply running samples for them. This can be challenging with many different research goals among the client labs and varying degrees of experience with flow cytometry and cell biology in general. The core facility that I run includes a number of instruments relevant to cellular isolation and analysis including the GentleMACS, AutoMACS pro and MACS Quant amongst other infrastructure. The scientific focus of the KRCBS ranges from cancer, cardiovascular disease, hematology, critical care and immunological research.

    Abstract:
    DATE: November 30, 2016
    TIME: 9:00 AM PT, 12:00PM ET


    When using a flow cytometry core facility, researchers commonly evaluate how to process their cellular samples most efficiently, while also ensuring the highest quality and reproducibility of their data. In this webinar, Chris Spring, from St. Michael’s Hospital Research Institute, Toronto, will discuss how Miltenyi Biotec’s line of instrumentation, including the gentleMACS™ Dissociator, autoMACS® Pro Separator and MACSQuant® Analyzer, has improved the performance and quality of research projects in his core facility. Furthermore, Chris will provide tips and tricks for optimizing sample preparation and experimental design for cell analysis that will help researchers get the most out of their flow analysis.  Some key topics include increased yield and reproducibility during tissue dissociation, efficient rare cell sorting through pre-enrichment and decreased time spent cleaning and maintaining instruments. If you are interested in learning more about best practices in optimizing sample processing for improved cell analysis, we cordially invite you to join the discussion with Chris Spring on Wednesday, November 30th at 9 am PST.

    Learning Objective 1: Learn about best practices in optimizing sample processing for improved flow cytometry

    Learning Objctive 2: Learn about tips and tricks for pre-enrichment of rare cell populations before flow sorting and analysis
     

    Show Resources
    You May Also Like
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    SEP 05, 2019 04:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 04:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    JUN 18, 2019 04:00 PM CEST
    C.E. CREDITS
    JUN 18, 2019 04:00 PM CEST
    DATE: June 18, 2019TIME: 7:00am PDT, 10:00 EDT, 4:00pm CET PSCs represent an important tool in a wide range of applications, including basic research, disease modeling, drug...
    Loading Comments...
    Show Resources