NOV 30, 2016 09:00 AM PST

Best practices in optimizing sample processing for improved flow cytometry

Speakers
  • Flow Cytometry Core Specialist, Keenan Research Centre for Biomedical Science, St. Michael's Research Hospital
    Biography
      I have been working in cell biology for 15 years, primarily working on analyzing the role of platelets in immunological and cardiovascular diseases. Most of my scientific career has been spent in laboratories in St Michael's Hospital, as well as McMaster University and the Australian Centre for Blood Diseases. I am currently the co-President of the Canadian Microscopy and Cytometry Association and have been involved with them since 2012.

      For the last 6 years I have run a flow cytometry and cell sorting core facility serving ~45 laboratories and 500 scientists in the Keenan Research Centre for Biomedical Science (KRCBS), St. Michael's Hospital. The core facility focuses on teaching individuals to do proper flow cytometry and consulting with scientists on their research programs rather than simply running samples for them. This can be challenging with many different research goals among the client labs and varying degrees of experience with flow cytometry and cell biology in general. The core facility that I run includes a number of instruments relevant to cellular isolation and analysis including the GentleMACS, AutoMACS pro and MACS Quant amongst other infrastructure. The scientific focus of the KRCBS ranges from cancer, cardiovascular disease, hematology, critical care and immunological research.

    Abstract:
    DATE: November 30, 2016
    TIME: 9:00 AM PT, 12:00PM ET


    When using a flow cytometry core facility, researchers commonly evaluate how to process their cellular samples most efficiently, while also ensuring the highest quality and reproducibility of their data. In this webinar, Chris Spring, from St. Michael’s Hospital Research Institute, Toronto, will discuss how Miltenyi Biotec’s line of instrumentation, including the gentleMACS™ Dissociator, autoMACS® Pro Separator and MACSQuant® Analyzer, has improved the performance and quality of research projects in his core facility. Furthermore, Chris will provide tips and tricks for optimizing sample preparation and experimental design for cell analysis that will help researchers get the most out of their flow analysis.  Some key topics include increased yield and reproducibility during tissue dissociation, efficient rare cell sorting through pre-enrichment and decreased time spent cleaning and maintaining instruments. If you are interested in learning more about best practices in optimizing sample processing for improved cell analysis, we cordially invite you to join the discussion with Chris Spring on Wednesday, November 30th at 9 am PST.

    Learning Objective 1: Learn about best practices in optimizing sample processing for improved flow cytometry

    Learning Objctive 2: Learn about tips and tricks for pre-enrichment of rare cell populations before flow sorting and analysis
     

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