FEB 04, 2016 6:00 AM PST

Zebrafish: an upswing model in research and the need for refinement of anaesthesia

Speaker
  • Post-doctoral Researcher, i3S (Instituto de Investigação e Inovação em Saúde)
    Biography
      Ana is a researcher with a degree in Biology from University of Coimbra (2005) and a PhD in Veterinarian Sciences from ICBAS, University of Porto (2012). Her research line started right after her graduation, in the Institute for Molecular and Cell Biology (IBMC), where she intended to understand if different depths of anaesthesia could induce behavioural changes in rodents. This research continued in her PhD and during one year as a post-doc in a project. The gap between the increasing use of zebrafish models and the lack of appropriate knowledge of some procedures in research, such as anaesthesia, led Ana to apply her research line to zebrafish. She was funded by FCT (Fundação para a Ciência e Tecnologia) as Principal Investigator of a project regarding the effects of anaesthesia in zebrafish development using behavioural tasks, and assessment of morphology, genetic expression, and cellular alterations (IBMC, UTAD, CNBC). Currently, she has got a post-doctoral fellowship to optimize zebrafish anaesthesia using a clinical and animal welfare perspective. Besides this line of research, she is also particularly interested in finding better euthanasia methods and refining other situations in laboratory animals. Since 2007 she is also a trainer in the Laboratory Animal Science course FELASA (category C) at IBMC.

    Abstract

         In the last decade the number of publications in zebrafish tripled, from 938 in 2005 to 2432 in 2015 (Pubmed database; keyword: “zebrafish” in Title/Abstract; 12/4/2015), being the animal model with the highest increase in publication. These numbers reflect the importance of zebrafish as a good practical and scientific model for several human diseases, drug discovery and gene function identification through behavioural phenomics and high-throughput genetic and molecular screening.

         Zebrafish is legally protected by the European Directive on the protection of animals used for scientific purposes (2010/63/EU) when they become capable of independent feeding.
         
         However, the increasing use of zebrafish has not been accompanied by the evolution of anaesthetic protocols suitable for different experimental situations. Ethyl 3-aminobenzoate methanesulfate (MS222) is the standard fish anaesthetic, but concerns have been raised concerning aversion/ stress induction and cardiovascular depression in long procedures. This lecture intends to provide a review of the anaesthetics used in zebrafish in different contexts and the proposal of new anaesthetic protocols. The drugs’ availability, preparation, clinical efficacy and safety, and animal welfare issues will be discussed.

         There are legal, scientific and animal welfare reasons to study procedures’ refinement in zebrafish. This animal model needs proper research in the area of laboratory animal science for a better care and scientific quality.


    Show Resources
    You May Also Like
    SEP 10, 2020 9:00 AM PDT
    C.E. CREDITS
    SEP 10, 2020 9:00 AM PDT
    Date: September 10, 2020 Time: 9:00am (PDT), 12:00pm (EDT) Osmolality testing is relevant throughout the entire bioprocessing workflow. As customers look to refine mAb and gene therapy workf...
    NOV 16, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 16, 2020 8:00 AM PST
    Date: November 16, 2020 Time: 8:00am (PST), 11:00am (EST) CRISPR screening has become the prime discovery tool in modern biomedical research and drug discovery. At the same time, most screen...
    NOV 18, 2020 8:00 AM PST
    C.E. CREDITS
    NOV 18, 2020 8:00 AM PST
    DATE: November 18, 2020 TIME: 08:00am PDT We develop and implement technologies to solve some of the major bottlenecks in biomedical research. In particular, we establish new imaging approac...
    NOV 10, 2020 7:00 AM PST
    C.E. CREDITS
    NOV 10, 2020 7:00 AM PST
    DATE: November 10, 2020 TIME: 7:00am PDT, 10:00am EDT Automation can provide tremendous benefits such as increased pipetting precision and accuracy, productivity, and throughput. Numerous wo...
    OCT 29, 2020 6:00 AM PDT
    C.E. CREDITS
    OCT 29, 2020 6:00 AM PDT
    Date: October 29, 2020 Time: 6:00am (PDT), 9:00am (EDT), Chronic inflammation can occur as a result of a combination of genetic predispositions and environmental factors. Epigenetic modifica...
    AUG 18, 2020 10:00 AM PDT
    C.E. CREDITS
    AUG 18, 2020 10:00 AM PDT
    DATE: August 18, 2020 TIME: 10:00am PT Get deeper understanding of gene expression patterns by using assays that retain spatial organization at single cell resolution! Come learn about the n...
    Loading Comments...
    Show Resources
    Attendees
    • See more