Companies / Amerigo Scientific / "4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one ( DFHBI-1T )"
Amerigo Scientific

"4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one ( DFHBI-1T )" | Amerigo Scientific

DFHBI-1T is a non-fluorescent dye that consists of an 1,1,1-trifluoroethyl substituent on the imidazolone ring of the DFHBI fluorophore. Upon binding to the Spinach™ aptamer family, DFHBI-1T is converted to a highly fluorescent state that can be detected at the emission wavelength of 505 nm. DFHBI-1T is cellpermeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags. DFHBI-1T has been found to have lower background fluorescence than DFHBI and exhibits an overall increase in brightness in living cells. Further, DFHBI-1T can also selectively detect tagged RNA in total RNA gel electrophoresis. Thus, bypassing the need for Northern blot analysis./

Data/

Live-cell

A. Live-cell imaging of a COS7 cell expressing CGG60-Spinach2™ in the presence of either 20 µM DFHBI or DFHBI-1T. Images of the same cell were acquired using a 100 msec exposure with a GFP filter set. B. Quantification of average DFHBI-1T brightness of 10 cells expressing CGG60-Spinach™ normalized to the brightness of DFHBI.

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ABOUT THE COMPANY

Amerigo Scientific is a distribution company focused on providing critical products and services to the biomedical and life science research communities. Although a new company, Amerigo Scientific's founder has had more than 20 years of rich experience in the biomedical and biochemical fields, and has established close contacts with key personnel in...
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