The Caspase-3/7 assay reagent is specially formulated for use in the Incucyte ® live-cell analysis system and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. In addition, the Caspase-3/7 assay reagent is non-perturbing to cell growth and morphology.