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    Antigen presenting cells

    Antigen presenting cells (APC) are a class of immune cells that mediate the immune response. APCs process foreign antigen and present the antigen to T cells, the immune cells with kill foreign cells. Several subsets of immune cells, including dendritic cells (DC) and macrophages are capable of serving as APCs.

    Webinars

    • MAR 28, 2024 | 8:00 AM
      Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
      Identification of Frequent Autoreactive B-cell Response in Pancreatic...
      B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this web...
      Speaker: Min Yao, Ph.D. , Patrick Murphy, Ph.D.
      Sponsored By: Sony Biotechnology
      12
      MAR 26, 2024 | 11:00 AM
      Complete and Comprehensive Biomarker Discovery: TerraFlow for Flow Cytometry Analysis
      Complete and Comprehensive Biomarker Discovery: TerraFlow for...
      Ever wonder what you’re missing in your data? The sheer complexity of today’s flow and mass cytometry datasets demands automated solutions. Machine learning plugins only provide...
      Speaker: Dan Freeman , Pratip K. Chattopadhyay
      Sponsored By: terraFlow Bio
      19
      On Demand
      C.E. CREDITS
      Proliferation Biomarkers in Drug Discovery and Development, The Immunoassay of Thymidine Kinase 1
      Proliferation Biomarkers in Drug Discovery and Development, The...
      Proliferation biomarkers are proteins that are up-regulated in growing cells. Immunological staining of tumor tissue can provide an indication of the aggressivity of the tumor and provide in...
      Speaker: Martin Shaw
      Presented at: Drug Discovery & Development Virtual Event Series 2024
      3
      NOV 07, 2023 | 10:00 AM
      Understanding the Mechanisms of Resistance in CAR T Immunotherapy for Lymphoma and Strategies to Overcome Them
      Understanding the Mechanisms of Resistance in CAR T Immunotherapy...
      In the last 5 years, the US Food and Drug Administration has approved 4 anti-CD19 chimeric antigen receptor T cell (CART19) products for relapsed/refractory B cell lymphomas and leukemia. Ho...
      Speaker: Marco Ruella
      Sponsored By: Beckman Coulter Life Sciences
      22
      JUL 26, 2023 | 11:20 AM
      CD3ζ as a novel integration site for reprogramming immune cells with Chimeric Antigen Receptors
      CD3ζ as a novel integration site for reprogramming immune cells...
      Chimeric antigen receptor (CAR)-reprogrammed immune cells offer exciting therapeutic potential for addressing oncology, autoimmune diseases, transplant medicine, and infections. However, cur...
      Speaker: Dr. Dimitrios L. Wagner
      Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
      4
      SEP 08, 2022 | 6:00 AM
      Transposon Mediated Forward Genetic Screens Identify Cell Intrinsic Resistance to Emerging Viral Pathogens
      Transposon Mediated Forward Genetic Screens Identify Cell Intrinsic...
      Recent outbreaks of Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have exposed our limited therapeutic options for such diseases and our poor understand...
      Speaker: Anna Bruchez, PhD
      Presented at: Microbiology Week Virtual Event Series 2022
      3
      SEP 08, 2022 | 6:00 AM
      Covid Testing Challenges And Opportunities;  A Picture is Worth a Thousand Words
      Covid Testing Challenges And Opportunities; A Picture is Worth...
      Since its discovery in late 2019 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created a global pandemic of coronavirus disease 2019 (COVID-19). As of July of 2022, mo...
      Speaker: Irene Bosch, PhD
      Presented at: Microbiology Week Virtual Event Series 2022
      3
      JUL 21, 2022 | 8:00 AM
      T cell analysis - Fine-tuning your T cell research workflow step by step
      T cell analysis - Fine-tuning your T cell research workflow step...
      Date: December 09, 2022 Time: 11:00am (PST), 2:00pm (EST), 8:00pm (CEST) Flow cytometry is a trusted method to monitor T cell phenotype, plasticity, and functionality in basic research as we...
      Speaker: Dr. Marcello Stein
      Sponsored By: Miltenyi Biotec
      13 1
      JUL 12, 2022 | 8:00 AM
      Solutions for dendritic cell research
      Solutions for dendritic cell research
      Date: July 12, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) Dendritic cells (DCs) are a heterogeneous population of myeloid immune cells that act as peripheral sentinels specializin...
      Speaker: Dr. Nizar Yousif
      Sponsored By: Miltenyi Biotec
      15
      MAY 12, 2022 | 8:00 AM
      T cell culture - Fine-tuning your T cell research workflow step by step
      T cell culture - Fine-tuning your T cell research workflow step...
      Date: May 12, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cell culture is key for efficient and reliable T cell workflows, be it activation/ and expansion, T helper cell polarizati...
      Speaker: Dr. Marcello Stein
      Sponsored By: Miltenyi Biotec
      17
      MAR 24, 2022 | 8:00 AM
      T cell separation: Fine-tuning your T cell research workflow step by step
      T cell separation: Fine-tuning your T cell research workflow step...
      Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
      Speaker: Marcello Stein, PhD.
      Sponsored By: Miltenyi Biotec
      6
      MAR 01, 2022 | 10:00 AM
      Impaired Dendritic Cell Homing in COVID-19
      Impaired Dendritic Cell Homing in COVID-19
      Date: March 01, 2022 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CEST) The high mortality of COVID-19 is mostly attributed to acute respiratory distress syndrome (ARDS), whose histopathologic...
      Speaker: Lukas Borcherding , Ralf Huss, MD, PhD
      Sponsored By: Visiopharm®
      10
      FEB 23, 2022 | 6:00 AM
      Autoantibodies as Biomarkers in Immuno-oncology
      Autoantibodies as Biomarkers in Immuno-oncology
      Cancer still accounts for nearly 1 in every 4 deaths worldwide. New immunotherapies have the power to induce durable responses in patients with fatal cancers, but only a small percentage of...
      Speaker: Hans-Dieter Zucht, PhD
      Presented at: Drug Discovery & Development Virtual Event Series 2022
      Sponsored By: Luminex - A DiaSorin Company
      10
      OCT 28, 2021 | 6:00 AM
      Role of TrimericS IgG Antibodies in monitoring the immune response of recipients of COVID-19 Vaccination
      Role of TrimericS IgG Antibodies in monitoring the immune response...
      Date: October 28, 2021 Time: 6:00am (PDT), 09:00am (EDT) COVID-19 has spurred the development of a plethora of tests from various manufacturers. While molecular tests and rapid antigen tests...
      Speaker: Prof. Dr. Ashok Rattan
      Sponsored By: Diasorin
      7
      OCT 26, 2021 | 6:00 AM
      IR Biotyper® - New insights into microorganism strain- and serotyping by FT-IR spectroscopy
      IR Biotyper® - New insights into microorganism strain- and serotyping...
      Date: October 26, 2021 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) With the IR Biotyper®, Dr. Markus Meyer will introduce the audience into the world of FT-IR (Fourier Transform Infr...
      Speaker: Maria Pardos de la Gandara, MD, PhD , Markus Meyer, PhD
      Sponsored By: Bruker Microbiology & Infection Diagnostics
      8
      OCT 21, 2021 | 8:00 AM
      Generating protein reagents to evaluate the antibody and CD8+ T cell responses to SARS-CoV-2
      Generating protein reagents to evaluate the antibody and CD8+...
      DATE: October 21, 2021 TIME: 8:00am PDT Coronavirus disease 2019 (COVID-19) is a global health crisis caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and th...
      Speaker: Natalia Guadalupe Herrera
      Sponsored By: Thermo Fisher Scientific/Gibco
      3
      OCT 13, 2021 | 12:00 AM
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 Evolution: Addition by Subtraction
      SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
      Speaker: Kevin R. McCarthy, PhD
      Presented at: Coronavirus Series
      0
      OCT 06, 2021 | 12:00 PM
      Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
      Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
      Advancements in immuno-oncology have been moving at an ever-quickening pace. From the development of new CAR-T therapies to a better understanding of the mechanism of action of monoclonal an...
      Speaker: Katie Gillis , Brian E. Hall , Stephanie Brunelle, Ph.D.
      Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
      Sponsored By: Luminex - A DiaSorin Company
      0
      OCT 06, 2021 | 9:00 AM
      Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
      Antibody titration for flow cytometric analysis of Extracellular...
      Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
      Speaker: Desmond Pink, PhD
      Sponsored By: Miltenyi Biotec
      6 1
      SEP 22, 2021 | 11:59 PM
      Measuring Chimeric Antigen T Cell Activation One Molecule at a Time
      Measuring Chimeric Antigen T Cell Activation One Molecule at a...
      T cells exquisitely discriminate between antigenic and self-peptide ligands presented on major histocompatibility complexes (pMHCs) despite subtle differences in affinities. This aspect of a...
      Speaker: Shalini Low-Nam, PhD
      Presented at: Cell Biology Virtual Event Series 2021
      1
      SEP 08, 2021 | 12:00 AM
      Development of Self-Replicating Propagation-Defective RNAs as Next Generation Vaccine Candidates Against Human Coronaviruses
      Development of Self-Replicating Propagation-Defective RNAs as...
      Self-amplifying RNA replicons are promising candidates for next generation vaccines against human coronaviruses with pandemic potential. Self-amplification of RNAs in host cells generates mo...
      Speaker: Isabel Sola, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      SEP 08, 2021 | 12:00 AM
      Memory CD4 T cells established by endemic human Coronaviruses have the potential to be recruited into the response to SARS-Cov-2
      Memory CD4 T cells established by endemic human Coronaviruses...
      For centuries, humans have become infected with endemic circulating Coronaviruses (hCoV), typically repeatedly throughout life. To understand how these respiratory infections establish CD4 T...
      Speaker: Andrea J. Sant, PhD
      Presented at: Microbiology Week Virtual Event Series 2021
      0
      JUL 22, 2021 | 5:00 PM
      Closing Keynote Presentation          Response to Second Generation CAR T Cell Therapy: It takes (at least) Two to Tango
      Closing Keynote Presentation Response to Second Generation...
      Though tumor targeting using chimeric receptors (CAR) engineered to bind tumor-associated surface proteins such as CD19 in B cells have demonstrated remarkable efficacy in cancer, this thera...
      Speaker: J Joseph Melenhorst, PhD
      Presented at: GenScript Gene & Cell Engineering Virtual Summit
      1
      JUN 22, 2021 | 10:00 AM
      Role of Cov-2 Serology in a vaccinated world
      Role of Cov-2 Serology in a vaccinated world
      Date: June 22, 2021 Time: 10:00am PST The objectives of this presentation are (1) to review current and emerging SARS-CoV-2 serology applications, (2) to discuss variation among different SA...
      Speaker: David M. Engman, MD, PhD
      Sponsored By: Diasorin
      25
    MAR 28, 2024 | 8:00 AM
    Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
    Identification of Frequent Autoreactive B-cell Response in Pancreatic...
    B cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this web...
    Speaker: Min Yao, Ph.D. , Patrick Murphy, Ph.D.
    Sponsored By: Sony Biotechnology
    12
    MAR 26, 2024 | 11:00 AM
    Complete and Comprehensive Biomarker Discovery: TerraFlow for Flow Cytometry Analysis
    Complete and Comprehensive Biomarker Discovery: TerraFlow for...
    Ever wonder what you’re missing in your data? The sheer complexity of today’s flow and mass cytometry datasets demands automated solutions. Machine learning plugins only provide...
    Speaker: Dan Freeman , Pratip K. Chattopadhyay
    Sponsored By: terraFlow Bio
    19
    On Demand
    C.E. CREDITS
    Proliferation Biomarkers in Drug Discovery and Development, The Immunoassay of Thymidine Kinase 1
    Proliferation Biomarkers in Drug Discovery and Development, The...
    Proliferation biomarkers are proteins that are up-regulated in growing cells. Immunological staining of tumor tissue can provide an indication of the aggressivity of the tumor and provide in...
    Speaker: Martin Shaw
    Presented at: Drug Discovery & Development Virtual Event Series 2024
    3
    NOV 07, 2023 | 10:00 AM
    Understanding the Mechanisms of Resistance in CAR T Immunotherapy for Lymphoma and Strategies to Overcome Them
    Understanding the Mechanisms of Resistance in CAR T Immunotherapy...
    In the last 5 years, the US Food and Drug Administration has approved 4 anti-CD19 chimeric antigen receptor T cell (CART19) products for relapsed/refractory B cell lymphomas and leukemia. Ho...
    Speaker: Marco Ruella
    Sponsored By: Beckman Coulter Life Sciences
    22
    JUL 26, 2023 | 11:20 AM
    CD3ζ as a novel integration site for reprogramming immune cells with Chimeric Antigen Receptors
    CD3ζ as a novel integration site for reprogramming immune cells...
    Chimeric antigen receptor (CAR)-reprogrammed immune cells offer exciting therapeutic potential for addressing oncology, autoimmune diseases, transplant medicine, and infections. However, cur...
    Speaker: Dr. Dimitrios L. Wagner
    Presented at: GenScript 3rd Annual Gene and Cell Engineering Summit
    4
     
     
  • SEP 08, 2022 | 6:00 AM
    Transposon Mediated Forward Genetic Screens Identify Cell Intrinsic Resistance to Emerging Viral Pathogens
    Transposon Mediated Forward Genetic Screens Identify Cell Intrinsic...
    Recent outbreaks of Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have exposed our limited therapeutic options for such diseases and our poor understand...
    Speaker: Anna Bruchez, PhD
    Presented at: Microbiology Week Virtual Event Series 2022
    3
    SEP 08, 2022 | 6:00 AM
    Covid Testing Challenges And Opportunities;  A Picture is Worth a Thousand Words
    Covid Testing Challenges And Opportunities; A Picture is Worth...
    Since its discovery in late 2019 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has created a global pandemic of coronavirus disease 2019 (COVID-19). As of July of 2022, mo...
    Speaker: Irene Bosch, PhD
    Presented at: Microbiology Week Virtual Event Series 2022
    3
    JUL 21, 2022 | 8:00 AM
    T cell analysis - Fine-tuning your T cell research workflow step by step
    T cell analysis - Fine-tuning your T cell research workflow step...
    Date: December 09, 2022 Time: 11:00am (PST), 2:00pm (EST), 8:00pm (CEST) Flow cytometry is a trusted method to monitor T cell phenotype, plasticity, and functionality in basic research as we...
    Speaker: Dr. Marcello Stein
    Sponsored By: Miltenyi Biotec
    13 1
    JUL 12, 2022 | 8:00 AM
    Solutions for dendritic cell research
    Solutions for dendritic cell research
    Date: July 12, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CEST) Dendritic cells (DCs) are a heterogeneous population of myeloid immune cells that act as peripheral sentinels specializin...
    Speaker: Dr. Nizar Yousif
    Sponsored By: Miltenyi Biotec
    15
    MAY 12, 2022 | 8:00 AM
    T cell culture - Fine-tuning your T cell research workflow step by step
    T cell culture - Fine-tuning your T cell research workflow step...
    Date: May 12, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Cell culture is key for efficient and reliable T cell workflows, be it activation/ and expansion, T helper cell polarizati...
    Speaker: Dr. Marcello Stein
    Sponsored By: Miltenyi Biotec
    17
     
     
  • MAR 24, 2022 | 8:00 AM
    T cell separation: Fine-tuning your T cell research workflow step by step
    T cell separation: Fine-tuning your T cell research workflow step...
    Date: March 24, 2021 Time: 08:00am (PDT), 11:00pm (EDT) Simply enough, we want to treat our cells well. Afterall, we want them to tell us their stories in basic research or work for us in th...
    Speaker: Marcello Stein, PhD.
    Sponsored By: Miltenyi Biotec
    6
    MAR 01, 2022 | 10:00 AM
    Impaired Dendritic Cell Homing in COVID-19
    Impaired Dendritic Cell Homing in COVID-19
    Date: March 01, 2022 Time: 10:00am (PST), 1:00pm (EST), 7:00pm (CEST) The high mortality of COVID-19 is mostly attributed to acute respiratory distress syndrome (ARDS), whose histopathologic...
    Speaker: Lukas Borcherding , Ralf Huss, MD, PhD
    Sponsored By: Visiopharm®
    10
    FEB 23, 2022 | 6:00 AM
    Autoantibodies as Biomarkers in Immuno-oncology
    Autoantibodies as Biomarkers in Immuno-oncology
    Cancer still accounts for nearly 1 in every 4 deaths worldwide. New immunotherapies have the power to induce durable responses in patients with fatal cancers, but only a small percentage of...
    Speaker: Hans-Dieter Zucht, PhD
    Presented at: Drug Discovery & Development Virtual Event Series 2022
    Sponsored By: Luminex - A DiaSorin Company
    10
    OCT 28, 2021 | 6:00 AM
    Role of TrimericS IgG Antibodies in monitoring the immune response of recipients of COVID-19 Vaccination
    Role of TrimericS IgG Antibodies in monitoring the immune response...
    Date: October 28, 2021 Time: 6:00am (PDT), 09:00am (EDT) COVID-19 has spurred the development of a plethora of tests from various manufacturers. While molecular tests and rapid antigen tests...
    Speaker: Prof. Dr. Ashok Rattan
    Sponsored By: Diasorin
    7
    OCT 26, 2021 | 6:00 AM
    IR Biotyper® - New insights into microorganism strain- and serotyping by FT-IR spectroscopy
    IR Biotyper® - New insights into microorganism strain- and serotyping...
    Date: October 26, 2021 Time: 6:00am (PDT), 9:00am (EDT), 3:00pm (CEST) With the IR Biotyper®, Dr. Markus Meyer will introduce the audience into the world of FT-IR (Fourier Transform Infr...
    Speaker: Maria Pardos de la Gandara, MD, PhD , Markus Meyer, PhD
    Sponsored By: Bruker Microbiology & Infection Diagnostics
    8
     
     
  • OCT 21, 2021 | 8:00 AM
    Generating protein reagents to evaluate the antibody and CD8+ T cell responses to SARS-CoV-2
    Generating protein reagents to evaluate the antibody and CD8+...
    DATE: October 21, 2021 TIME: 8:00am PDT Coronavirus disease 2019 (COVID-19) is a global health crisis caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and th...
    Speaker: Natalia Guadalupe Herrera
    Sponsored By: Thermo Fisher Scientific/Gibco
    3
    OCT 13, 2021 | 12:00 AM
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 Evolution: Addition by Subtraction
    SARS-CoV-2 is an emergent virus. Specifically how it will adapt to human hosts and populations with growing immunity is unknown. Coronaviruses acquire substitutions more slowly than other RN...
    Speaker: Kevin R. McCarthy, PhD
    Presented at: Coronavirus Series
    0
    OCT 06, 2021 | 12:00 PM
    Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
    Panel Presentation: Luminex Imaging and Flow Cytometry in Immuno-Oncology
    Advancements in immuno-oncology have been moving at an ever-quickening pace. From the development of new CAR-T therapies to a better understanding of the mechanism of action of monoclonal an...
    Speaker: Katie Gillis , Brian E. Hall , Stephanie Brunelle, Ph.D.
    Presented at: Cancer Research & Oncology Week Virtual Event Series 2021
    Sponsored By: Luminex - A DiaSorin Company
    0
    OCT 06, 2021 | 9:00 AM
    Antibody titration for flow cytometric analysis of Extracellular Vesicles: Are cell based protocols up to the task?
    Antibody titration for flow cytometric analysis of Extracellular...
    Date: October 6, 2021 Time: 9:00am (PDT), 12:00pm (EDT) ANTIBODY TITRATION is critical to the success of any flow cytometry experiment. This is even more so in microflow cytometric analysis...
    Speaker: Desmond Pink, PhD
    Sponsored By: Miltenyi Biotec
    6 1
    SEP 22, 2021 | 11:59 PM
    Measuring Chimeric Antigen T Cell Activation One Molecule at a Time
    Measuring Chimeric Antigen T Cell Activation One Molecule at a...
    T cells exquisitely discriminate between antigenic and self-peptide ligands presented on major histocompatibility complexes (pMHCs) despite subtle differences in affinities. This aspect of a...
    Speaker: Shalini Low-Nam, PhD
    Presented at: Cell Biology Virtual Event Series 2021
    1
     
     
  • SEP 08, 2021 | 12:00 AM
    Development of Self-Replicating Propagation-Defective RNAs as Next Generation Vaccine Candidates Against Human Coronaviruses
    Development of Self-Replicating Propagation-Defective RNAs as...
    Self-amplifying RNA replicons are promising candidates for next generation vaccines against human coronaviruses with pandemic potential. Self-amplification of RNAs in host cells generates mo...
    Speaker: Isabel Sola, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    SEP 08, 2021 | 12:00 AM
    Memory CD4 T cells established by endemic human Coronaviruses have the potential to be recruited into the response to SARS-Cov-2
    Memory CD4 T cells established by endemic human Coronaviruses...
    For centuries, humans have become infected with endemic circulating Coronaviruses (hCoV), typically repeatedly throughout life. To understand how these respiratory infections establish CD4 T...
    Speaker: Andrea J. Sant, PhD
    Presented at: Microbiology Week Virtual Event Series 2021
    0
    JUL 22, 2021 | 5:00 PM
    Closing Keynote Presentation          Response to Second Generation CAR T Cell Therapy: It takes (at least) Two to Tango
    Closing Keynote Presentation Response to Second Generation...
    Though tumor targeting using chimeric receptors (CAR) engineered to bind tumor-associated surface proteins such as CD19 in B cells have demonstrated remarkable efficacy in cancer, this thera...
    Speaker: J Joseph Melenhorst, PhD
    Presented at: GenScript Gene & Cell Engineering Virtual Summit
    1
    JUN 22, 2021 | 10:00 AM
    Role of Cov-2 Serology in a vaccinated world
    Role of Cov-2 Serology in a vaccinated world
    Date: June 22, 2021 Time: 10:00am PST The objectives of this presentation are (1) to review current and emerging SARS-CoV-2 serology applications, (2) to discuss variation among different SA...
    Speaker: David M. Engman, MD, PhD
    Sponsored By: Diasorin
    25
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