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    Bioprocessing

    Bioprocessing: The use of biological (typically cellular or enzymatic) processes to affect desired physical or chemical changes, especially in various industrial applications; (also) the processing of organic or inorganic materials (coal, minerals, etc.) using microbiological methods.

    Webinars

    • OCT 24, 2024 | 8:00 AM
      From Data to Discovery: Transforming Biotherapeutic Analysis with Next-Generation Workflows
      From Data to Discovery: Transforming Biotherapeutic Analysis with...
      Join us as we unveil the latest enhancements to our comprehensive suite of workflows designed to transform and streamline the analysis of biotherapeutics. Discover cutting-edge advancements...
      Speaker: Dr. Sara Carillo, PhD , Roberto Gamez
      Sponsored By: Thermo Fisher Scientific
      12
      On Demand
      The Importance of a Serum-Free, Chemically-Defined Manufacturing Workflow for T Cell Therapy
      The Importance of a Serum-Free, Chemically-Defined Manufacturing...
      Cell-based therapies are advanced medical treatments for cancers and other diseases. Autologous and allogeneic cell therapies use immune cells from either a patient or from healthy donor sam...
      Speaker: Sam Bhattacharya, PhD, MBA
      Presented at: Immuno-Oncology Virtual Event Series 2024
      2
      APR 03, 2024 | 9:00 AM
      C.E. CREDITS
      Keynote Presentation: Mammalian Cell Systems for Production of Therapeutic Proteins and Carbohydrates- Advances in Cell Line Development and Bioprocessing with Live Q&A
      Keynote Presentation: Mammalian Cell Systems for Production of...
      Cultured mammalian cells, particularly Chinese hamster ovary (CHO) cells are widely used in the biotechnology industry for production of a wide range of therapeutics, predominantly monoclona...
      Speaker: Susan Sharfstein
      Presented at: Bioprocessing Virtual Event Series 2024
      3
      On Demand
      C.E. CREDITS
      Empowering Process Development for Viral Vector-Based Medicines
      Empowering Process Development for Viral Vector-Based Medicines
      Effective process development for viral vector-based medicines is paramount for optimized process efficiency, robustness, scalability, and product quality. Well-designed processes streamline...
      Speaker: Matthias Müllner, PhD
      Presented at: Bioprocessing Virtual Event Series 2024
      3
      On Demand
      C.E. CREDITS
      Application of AI and Digital Twins for Bioprocessing: Pitfalls and Solution Paths for Accelerated Process Development
      Application of AI and Digital Twins for Bioprocessing: Pitfalls...
      In the slowly evolving landscape of bioprocess development and manufacturing, digital bioprocess-twins have emerged as potential accelerators. While advanced algorithms are at the heart of t...
      Speaker: Mark Duerkop, PhD
      Presented at: Bioprocessing Virtual Event Series 2024
      3
      MAR 28, 2024 | 8:00 AM
      Development of an Automated Online Flow Cytometry Method to Quantify Cell  Concentration and Fingerprint Bacterial Communities
      Development of an Automated Online Flow Cytometry Method to Quantify...
      Flow cytometry is a powerful method for the quantification of microorganisms, unparalleled by other technologies. We will (1) give an overview of the benefits and use cases of flow cytometry...
      Speaker: Konstanze Schiessl PhD , Juan López Gálvez
      Sponsored By: Beckman Coulter Life Sciences
      14
      NOV 15, 2023 | 10:00 AM
      Untangling Automated Proteomics
      Untangling Automated Proteomics
      Join our webinar to discover how automated sample preparation can free your lab from the time-consuming, costly, and complex manual workflows in proteomics. In the ever-evolving field of pro...
      Speaker: Dr. Sudha Savant
      Sponsored By: Beckman Coulter Life Sciences
      10
      OCT 16, 2023 | 9:00 AM
      Robust and Swift: Efficient One-Step Clarification Methods for AAV & LV Harvests
      Robust and Swift: Efficient One-Step Clarification Methods for...
      Clarification of mammalian and bacterial cell broths can be challenging when it comes to managing filter capacity and maximizing product recovery. Body feed filtration is an innovative techn...
      Speaker: Frederik Meierrieks , Andreas Pickl, PhD , Jennifer Labisch, PhD
      Sponsored By: Sartorius
      9
      SEP 20, 2023 | 9:00 AM
      Integration of Affinité's SPR technology for quality control in AAV bioproduction
      Integration of Affinité's SPR technology for quality control in...
      Adeno-associated virus (AAV) are increasingly produced as they hold tremendous potential in gene therapy. At research level, small quantities are produced for proof of concept studies. Howev...
      Speaker: Dr. Marie-Eve Paquet , Dr. Shuo Zhao , Dr Alina Venereo Sanchez
      Sponsored By: Affinité Instruments
      10
      APR 05, 2023 | 1:30 PM
      Introducing ALiCE® - Simple, scalable, cell-free expression of difficult-to-express proteins with Live Q&A
      Introducing ALiCE® - Simple, scalable, cell-free expression of...
      Many commercially promising proteins are difficult-to-express (DTE) in living cells and are lost before an industrial production process can be established. Cell-free protein expression (CFP...
      Speaker: Charles Williams, PhD
      Presented at: Bioprocessing Virtual Event Series 2023
      Sponsored By: LenioBio GmbH
      2
      APR 05, 2023 | 1:15 PM
      Panel Presentation: N-GLYcanyzer: An Automated Process Analytical Platform To Enable Continuous Manufacturing Of Biologics
      Panel Presentation: N-GLYcanyzer: An Automated Process Analytical...
      The biopharmaceutical industry is transitioning towards continuous biomanufacturing processes that require advanced analytical tools to monitor and control bioprocesses to produce high-quali...
      Speaker: Shishir P. S. Chundawat, PhD , Áron Györgypál, PhD
      Presented at: Bioprocessing Virtual Event Series 2023
      2
      APR 05, 2023 | 6:00 AM
      The Importance of Digital Lab Platforms and Why Bioprocessing Facilities Need to Adapt Them with Live Q&A
      The Importance of Digital Lab Platforms and Why Bioprocessing...
      Imagine, all of your lab work, your experimental data, your sample information and lineage tracing, your protocols and inventory management tools, all living on one, cohesive platform. What...
      Speaker: Tyler Gurney, MS
      Presented at: Bioprocessing Virtual Event Series 2023
      Sponsored By: eLabNext
      3
      APR 05, 2023 | 5:30 AM
      3D Visualisation and Characterisation of Downstream Bioprocessing Structures to Inform Advanced Designs
      3D Visualisation and Characterisation of Downstream Bioprocessing...
      High-resolution, 3D imaging enabled depth filters, chromatography columns and sterile filters to be analysed across multiple length scales. Generating a geometry-based database enabled struc...
      Speaker: Thomas F. Johnson, PhD
      Presented at: Bioprocessing Virtual Event Series 2023
      2
      MAR 01, 2023 | 8:00 AM
      Bioprocess Autosampler - Improve data quality, decrease effort
      Bioprocess Autosampler - Improve data quality, decrease effort
      Date: March 01, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Eppendorf is launching the Bioprocess Autosampler! This new addition to our product offering helps you gain more data wit...
      Speaker: Alwin Albers , Sonja Steinsiek
      Sponsored By: Eppendorf
      8
      DEC 07, 2022 | 8:00 AM
      Automation & Optimization Technology for Bioprocessing Operations
      Automation & Optimization Technology for Bioprocessing Operations
      Date: December 7, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Join Tom Fletcher, Scientific Director, Research and Development, and Guy Matthews, Director of Single Use Technologie...
      Speaker: Tom Fletcher , Guy Matthews
      Sponsored By: FUJIFILM Irvine Scientific
      16
      OCT 13, 2022 | 9:00 AM
      Smart EPU with Embedded CryoSPARC™ Live: integrated real-time data analysis for high-quality 3D protein structures
      Smart EPU with Embedded CryoSPARC™ Live: integrated real-time...
      First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
      Speaker: Julio Ortiz , Edward Pryor, PhD , Ali Punjani
      Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
      Sponsored By: Thermo Fisher Scientific
      10
      AUG 10, 2022 | 10:00 AM
      Avoiding hurdles in molecular assay development
      Avoiding hurdles in molecular assay development
      Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
      Speaker: Naeem Nathoo
      Sponsored By: Thermo Fisher Scientific
      16
      JUN 22, 2022 | 8:00 AM
      Delivering Improved Bioprocessing Workflows: The Manufacturing Science and Technology (MSAT) Service
      Delivering Improved Bioprocessing Workflows: The Manufacturing...
      Date: June 22, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Join us as we discuss the new Manufacturing Science and Technology (MSAT) Service offering, created in response to the nee...
      Speaker: Tom Fletcher , Michael De La Cruz, Ph.D.
      Sponsored By: FUJIFILM Irvine Scientific
      11
      MAY 18, 2022 | 9:00 AM
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
      Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. The ability to swiftly develop novel therapies often depends...
      Speaker: Steve Lewis , Jeff Hou, PhD
      Presented at: Laboratory Automation Virtual Event Series 2022
      Sponsored By: Thermo Fisher Scientific
      1
      APR 20, 2022 | 12:00 PM
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
      Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
      Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. The ability to swiftly develop novel therapies often depends...
      Speaker: Steve Lewis , Jeff Hou, PhD
      Presented at: Genetics Week Virtual Event Series 2022
      Sponsored By: Thermo Fisher Scientific
      1
      APR 06, 2022 | 11:30 AM
      Keynote Presentation: A Generic Manufacturing Platform for Downstream Processing on Non-mab Proteins
      Keynote Presentation: A Generic Manufacturing Platform for Downstream...
      The vast structural diversity of proteins is responsible for their functional versatility, but also makes their expression and purification a demanding task. Tagging proteins with affinity t...
      Speaker: Alois Jungbauer, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      1
      APR 06, 2022 | 9:00 AM
      Keynote Presentation: Continuous Bioprocessing - New Challenges and Opportunities for Membrane Technology
      Keynote Presentation: Continuous Bioprocessing - New Challenges...
      There is growing interest in the development of integrated continuous bioprocesses for the production of important biotherapeutics due to the potential for enhanced productivity, greater fle...
      Speaker: Andrew L. Zydney, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      1
      APR 06, 2022 | 5:30 AM
      In-Silico Scale-Up: Reduce Your Experimental Effort with Reactor Simulation
      In-Silico Scale-Up: Reduce Your Experimental Effort with Reactor...
      The design, optimization and troubleshooting of industrial bioprocesses are still largely based on empirical knowledge. Thus, the goal and motivation is to base these processes on a comprehe...
      Speaker: Christian Witz, PhD
      Presented at: Bioprocessing Virtual Event Series 2022
      1
      APR 06, 2022 | 5:30 AM
      Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing Cell Culture Process
      Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing...
      Biopharmaceuticals produced with mammalian cells are the key drivers for the medication of former untreatable diseases. The development of their production processes is still challenging bec...
      Speaker: Prof. Dr.-Ing. Habil. Ralf Pörtner
      Presented at: Bioprocessing Virtual Event Series 2022
      1
    OCT 24, 2024 | 8:00 AM
    From Data to Discovery: Transforming Biotherapeutic Analysis with Next-Generation Workflows
    From Data to Discovery: Transforming Biotherapeutic Analysis with...
    Join us as we unveil the latest enhancements to our comprehensive suite of workflows designed to transform and streamline the analysis of biotherapeutics. Discover cutting-edge advancements...
    Speaker: Dr. Sara Carillo, PhD , Roberto Gamez
    Sponsored By: Thermo Fisher Scientific
    12
    On Demand
    The Importance of a Serum-Free, Chemically-Defined Manufacturing Workflow for T Cell Therapy
    The Importance of a Serum-Free, Chemically-Defined Manufacturing...
    Cell-based therapies are advanced medical treatments for cancers and other diseases. Autologous and allogeneic cell therapies use immune cells from either a patient or from healthy donor sam...
    Speaker: Sam Bhattacharya, PhD, MBA
    Presented at: Immuno-Oncology Virtual Event Series 2024
    2
    APR 03, 2024 | 9:00 AM
    C.E. CREDITS
    Keynote Presentation: Mammalian Cell Systems for Production of Therapeutic Proteins and Carbohydrates- Advances in Cell Line Development and Bioprocessing with Live Q&A
    Keynote Presentation: Mammalian Cell Systems for Production of...
    Cultured mammalian cells, particularly Chinese hamster ovary (CHO) cells are widely used in the biotechnology industry for production of a wide range of therapeutics, predominantly monoclona...
    Speaker: Susan Sharfstein
    Presented at: Bioprocessing Virtual Event Series 2024
    3
    On Demand
    C.E. CREDITS
    Empowering Process Development for Viral Vector-Based Medicines
    Empowering Process Development for Viral Vector-Based Medicines
    Effective process development for viral vector-based medicines is paramount for optimized process efficiency, robustness, scalability, and product quality. Well-designed processes streamline...
    Speaker: Matthias Müllner, PhD
    Presented at: Bioprocessing Virtual Event Series 2024
    3
    On Demand
    C.E. CREDITS
    Application of AI and Digital Twins for Bioprocessing: Pitfalls and Solution Paths for Accelerated Process Development
    Application of AI and Digital Twins for Bioprocessing: Pitfalls...
    In the slowly evolving landscape of bioprocess development and manufacturing, digital bioprocess-twins have emerged as potential accelerators. While advanced algorithms are at the heart of t...
    Speaker: Mark Duerkop, PhD
    Presented at: Bioprocessing Virtual Event Series 2024
    3
     
     
  • MAR 28, 2024 | 8:00 AM
    Development of an Automated Online Flow Cytometry Method to Quantify Cell  Concentration and Fingerprint Bacterial Communities
    Development of an Automated Online Flow Cytometry Method to Quantify...
    Flow cytometry is a powerful method for the quantification of microorganisms, unparalleled by other technologies. We will (1) give an overview of the benefits and use cases of flow cytometry...
    Speaker: Konstanze Schiessl PhD , Juan López Gálvez
    Sponsored By: Beckman Coulter Life Sciences
    14
    NOV 15, 2023 | 10:00 AM
    Untangling Automated Proteomics
    Untangling Automated Proteomics
    Join our webinar to discover how automated sample preparation can free your lab from the time-consuming, costly, and complex manual workflows in proteomics. In the ever-evolving field of pro...
    Speaker: Dr. Sudha Savant
    Sponsored By: Beckman Coulter Life Sciences
    10
    OCT 16, 2023 | 9:00 AM
    Robust and Swift: Efficient One-Step Clarification Methods for AAV & LV Harvests
    Robust and Swift: Efficient One-Step Clarification Methods for...
    Clarification of mammalian and bacterial cell broths can be challenging when it comes to managing filter capacity and maximizing product recovery. Body feed filtration is an innovative techn...
    Speaker: Frederik Meierrieks , Andreas Pickl, PhD , Jennifer Labisch, PhD
    Sponsored By: Sartorius
    9
    SEP 20, 2023 | 9:00 AM
    Integration of Affinité's SPR technology for quality control in AAV bioproduction
    Integration of Affinité's SPR technology for quality control in...
    Adeno-associated virus (AAV) are increasingly produced as they hold tremendous potential in gene therapy. At research level, small quantities are produced for proof of concept studies. Howev...
    Speaker: Dr. Marie-Eve Paquet , Dr. Shuo Zhao , Dr Alina Venereo Sanchez
    Sponsored By: Affinité Instruments
    10
    APR 05, 2023 | 1:30 PM
    Introducing ALiCE® - Simple, scalable, cell-free expression of difficult-to-express proteins with Live Q&A
    Introducing ALiCE® - Simple, scalable, cell-free expression of...
    Many commercially promising proteins are difficult-to-express (DTE) in living cells and are lost before an industrial production process can be established. Cell-free protein expression (CFP...
    Speaker: Charles Williams, PhD
    Presented at: Bioprocessing Virtual Event Series 2023
    Sponsored By: LenioBio GmbH
    2
     
     
  • APR 05, 2023 | 1:15 PM
    Panel Presentation: N-GLYcanyzer: An Automated Process Analytical Platform To Enable Continuous Manufacturing Of Biologics
    Panel Presentation: N-GLYcanyzer: An Automated Process Analytical...
    The biopharmaceutical industry is transitioning towards continuous biomanufacturing processes that require advanced analytical tools to monitor and control bioprocesses to produce high-quali...
    Speaker: Shishir P. S. Chundawat, PhD , Áron Györgypál, PhD
    Presented at: Bioprocessing Virtual Event Series 2023
    2
    APR 05, 2023 | 6:00 AM
    The Importance of Digital Lab Platforms and Why Bioprocessing Facilities Need to Adapt Them with Live Q&A
    The Importance of Digital Lab Platforms and Why Bioprocessing...
    Imagine, all of your lab work, your experimental data, your sample information and lineage tracing, your protocols and inventory management tools, all living on one, cohesive platform. What...
    Speaker: Tyler Gurney, MS
    Presented at: Bioprocessing Virtual Event Series 2023
    Sponsored By: eLabNext
    3
    APR 05, 2023 | 5:30 AM
    3D Visualisation and Characterisation of Downstream Bioprocessing Structures to Inform Advanced Designs
    3D Visualisation and Characterisation of Downstream Bioprocessing...
    High-resolution, 3D imaging enabled depth filters, chromatography columns and sterile filters to be analysed across multiple length scales. Generating a geometry-based database enabled struc...
    Speaker: Thomas F. Johnson, PhD
    Presented at: Bioprocessing Virtual Event Series 2023
    2
    MAR 01, 2023 | 8:00 AM
    Bioprocess Autosampler - Improve data quality, decrease effort
    Bioprocess Autosampler - Improve data quality, decrease effort
    Date: March 01, 2023 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Eppendorf is launching the Bioprocess Autosampler! This new addition to our product offering helps you gain more data wit...
    Speaker: Alwin Albers , Sonja Steinsiek
    Sponsored By: Eppendorf
    8
    DEC 07, 2022 | 8:00 AM
    Automation & Optimization Technology for Bioprocessing Operations
    Automation & Optimization Technology for Bioprocessing Operations
    Date: December 7, 2022 Time: 8:00am (PST), 11:00am (EST), 5:00pm (CET) Join Tom Fletcher, Scientific Director, Research and Development, and Guy Matthews, Director of Single Use Technologie...
    Speaker: Tom Fletcher , Guy Matthews
    Sponsored By: FUJIFILM Irvine Scientific
    16
     
     
  • OCT 13, 2022 | 9:00 AM
    Smart EPU with Embedded CryoSPARC™ Live: integrated real-time data analysis for high-quality 3D protein structures
    Smart EPU with Embedded CryoSPARC™ Live: integrated real-time...
    First Broadcast: Date: October 12, 2022 Time: 8:00am PDT, 11:00am EDT Second Broadcast: Date: October 12, 2022 Time: 9:00am SGT The new Embedded CryoSPARC Live, now fully integrated with t...
    Speaker: Julio Ortiz , Edward Pryor, PhD , Ali Punjani
    Presented at: Thermo Fisher Scientific - Electron Microscopy Webinars
    Sponsored By: Thermo Fisher Scientific
    10
    AUG 10, 2022 | 10:00 AM
    Avoiding hurdles in molecular assay development
    Avoiding hurdles in molecular assay development
    Date: August 10, 2022 Time: 10:00am PDT, 1:00pm EDT The global pandemic has increased focus and scrutiny on molecular diagnostic assay development, resulting in a need for assays that provid...
    Speaker: Naeem Nathoo
    Sponsored By: Thermo Fisher Scientific
    16
    JUN 22, 2022 | 8:00 AM
    Delivering Improved Bioprocessing Workflows: The Manufacturing Science and Technology (MSAT) Service
    Delivering Improved Bioprocessing Workflows: The Manufacturing...
    Date: June 22, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Join us as we discuss the new Manufacturing Science and Technology (MSAT) Service offering, created in response to the nee...
    Speaker: Tom Fletcher , Michael De La Cruz, Ph.D.
    Sponsored By: FUJIFILM Irvine Scientific
    11
    MAY 18, 2022 | 9:00 AM
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
    Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. The ability to swiftly develop novel therapies often depends...
    Speaker: Steve Lewis , Jeff Hou, PhD
    Presented at: Laboratory Automation Virtual Event Series 2022
    Sponsored By: Thermo Fisher Scientific
    1
    APR 20, 2022 | 12:00 PM
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's Unique Approach to Rapidly Advancing Your Antibody and Protein Therapeutics
    Panel Presentation: Concept-to-Commercialization: Thermo Fisher's...
    Rapid screening and speed of scale-up in protein therapeutics are critical factors in today’s biotech and pharma workflows. The ability to swiftly develop novel therapies often depends...
    Speaker: Steve Lewis , Jeff Hou, PhD
    Presented at: Genetics Week Virtual Event Series 2022
    Sponsored By: Thermo Fisher Scientific
    1
     
     
  • APR 06, 2022 | 11:30 AM
    Keynote Presentation: A Generic Manufacturing Platform for Downstream Processing on Non-mab Proteins
    Keynote Presentation: A Generic Manufacturing Platform for Downstream...
    The vast structural diversity of proteins is responsible for their functional versatility, but also makes their expression and purification a demanding task. Tagging proteins with affinity t...
    Speaker: Alois Jungbauer, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    1
    APR 06, 2022 | 9:00 AM
    Keynote Presentation: Continuous Bioprocessing - New Challenges and Opportunities for Membrane Technology
    Keynote Presentation: Continuous Bioprocessing - New Challenges...
    There is growing interest in the development of integrated continuous bioprocesses for the production of important biotherapeutics due to the potential for enhanced productivity, greater fle...
    Speaker: Andrew L. Zydney, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    1
    APR 06, 2022 | 5:30 AM
    In-Silico Scale-Up: Reduce Your Experimental Effort with Reactor Simulation
    In-Silico Scale-Up: Reduce Your Experimental Effort with Reactor...
    The design, optimization and troubleshooting of industrial bioprocesses are still largely based on empirical knowledge. Thus, the goal and motivation is to base these processes on a comprehe...
    Speaker: Christian Witz, PhD
    Presented at: Bioprocessing Virtual Event Series 2022
    1
    APR 06, 2022 | 5:30 AM
    Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing Cell Culture Process
    Model-Assisted Design, Transfer and Scale-Up of an Antibody-Producing...
    Biopharmaceuticals produced with mammalian cells are the key drivers for the medication of former untreatable diseases. The development of their production processes is still challenging bec...
    Speaker: Prof. Dr.-Ing. Habil. Ralf Pörtner
    Presented at: Bioprocessing Virtual Event Series 2022
    1
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