DEC 09, 2021 9:00 AM PST

GenElute™-E Kits for Sustainable Nucleic Acid Purification

SPONSORED BY: MilliporeSigma

Nucleic acid purification is a common technique employed by numerous labs, including academic, pharmaceutical, agricultural, and many more. The combined costs of disposable reagents and the associated waste accumulation often contribute to significant operational costs for the lab and possibly the scope or scale of the completed work.

Traditional nucleic acid purification technologies use silica membrane columns to separate the DNA and RNA from the various sample types. In addition, high concentrations of chaotropic salts are used to bind the nucleic acids, including guanidine hydrochloride. The incorporation of numerous bind-and-wash steps may not only negatively affect the quantification of the DNA and RNA but also downstream applications, including PCR. GenElute™-E Single Spin nucleic acid purification kits are designed for simplified, rapid DNA and RNA purification workflows that result in a high-quality yield, without the resulting consumable and chemical wastes associated with traditional nucleic acid purification kits.

GenElute™-E Single Spin DNA and RNA purification kits incorporate the principle of negative chromatography using size exclusion to capture impurities, including organic solvents, proteins, salt, and detergent, while the nucleic acid flows through the column and is subsequently collected. Importantly, both waste and purified DNA and RNA are isolated in a single centrifugation step, enabling a 55% reduction of plastic material waste compared to silica-based kits. The reduction in tubes, pipette tips, and hazardous liquid wastes from traditional bind-and-wash steps and the convenience benefits of the GenElute™-E Single Spin kits save scientists time and resources with less handling and reduced waste.  

Purified nucleic acid workflows often involve multiple sample types and are required for numerous downstream applications. The sample type in which the nucleic acid is purified may present unique challenges including, effective lysis of the tissue, debris removal, and the presence of endogenous molecules that may inhibit downstream PCR and overall data quality. GenElute™-E Single Spin DNA and RNA purification kits are suitable for rapid purification of nucleic acids from diverse sources, including tissue, blood, cell, and have been rigorously evaluated for consistent PCR application performance. Explore the benefits of GenElute™-E technology and discover how you can improve the quality of your samples while sustainably saving time and resources as we work towards greener labs together.

About the Sponsor
MilliporeSigma is a global science and technology company determined to solve the toughest problems in life science. Our tools, services, and digital platforms empower scientists and engineers at every stage, helping to deliver scientific breakthroughs.
You May Also Like
JAN 28, 2022
Genetics & Genomics
Many Pathogenic Genetic Variants Don't Seem to Carry A Big Risk of Disease
JAN 28, 2022
Many Pathogenic Genetic Variants Don't Seem to Carry A Big Risk of Disease
For many years, a genetic mutation was usually linked to a serious disease. Researchers were able to connect errors in s ...
JAN 31, 2022
Health & Medicine
Genetic Risk Factor Identified for COVID-19 Loss of Smell
JAN 31, 2022
Genetic Risk Factor Identified for COVID-19 Loss of Smell
Scientists have identified a common genetic risk factor for the loss of smell due to COVID-19. Six months after testing ...
MAR 22, 2022
Cell & Molecular Biology
Self-Replicating RNA Molecules Show How Life May Have Arose
MAR 22, 2022
Self-Replicating RNA Molecules Show How Life May Have Arose
Cells use the genetic information contained in DNA to generate proteins, which carry out many of the processes of life. ...
MAR 26, 2022
Genetics & Genomics
Tumor Microvesicles Can Spread Mutated DNA
MAR 26, 2022
Tumor Microvesicles Can Spread Mutated DNA
The growth of tumors depends in part on its microenvironment, a dynamic area surrounding the tumor, which can include no ...
MAR 28, 2022
Cell & Molecular Biology
Scientists Reverse Aging in a Mouse Model
MAR 28, 2022
Scientists Reverse Aging in a Mouse Model
Time takes a toll on the body, and as we age, disease becomes more likely. Our bodies are not as good at fighting pathog ...
MAY 01, 2022
Genetics & Genomics
This Genetically Diverse Superbug Can Also Jump Species
MAY 01, 2022
This Genetically Diverse Superbug Can Also Jump Species
Two new studies have revealed more about a bacterial pathogen that can infect the gut, clostridium difficile. There are ...
Loading Comments...