AUG 04, 2018 10:53 AM PDT

The Viral Link to Irritable Bowel Disease

WRITTEN BY: Carmen Leitch

Inflammatory bowel disease (IBD) describes a couple of conditions in which the digestive tract does not function properly due to chronic inflammation. The gastrointestinal tract has been the subject of a lot of recent research. We have learned a lot about the microbiome, the community of microbes that resides there, helping us digest food and exerting a significant impact on our health. Researchers have looked to the microbiome to see how it is connected to IBD. New work reported in Nature Microbiology by scientists at North Carolina State University, the University of Texas Southwestern Medical Center and the University of Colorado has shown that in a rodent model of IBD, there is a major disruption in the microbiome - but not the part you might be expecting. These scientists were looking at viruses.

"We wanted to see how virus and bacterial communities differ in disease and healthy conditions," said Manuel Kleiner, a microbiologist at NC State. Kleiner was interested in bacteriophages, viruses that infect and can kill bacteria. While the trillions of bacteria in the microbiome are a focus of research, bacteriophages actually outnumber them and, the investigators speculated, could be causing inflammation.

"Phage numbers are elevated at the intestinal mucosal surface and increase in abundance during inflammatory bowel disease, suggesting that phages play an unidentified role in IBD," said the lead author of the report, Breck Duerkop, assistant professor of immunology and microbiology at the University of Colorado School of Medicine.

Gut inflammation is a hallmark of IBD. In Crohn’s disease, the lining of the tract is inflamed, and deeper tissues can be affected. In ulcerative colitis, it's the inner lining of the colon and intestine that is chronically inflamed.
 
The investigators used metagenomics tools to assess the bacteriophages that were present in the intestines of healthy mice and mice with inflamed intestinal tracts. They were unsurprised to find that communities of phages were stable and remained roughly comparable over time. Bacterial strain changes often could be associated with phage changes. In the diseased mice, however, the viral microbiomes became increasingly different from those of both healthy and other diseased mice.  

"Diseased mice showed individual divergent - seemingly random - increases in the abundance of some bacteriophages that are not associated with disease-causing bacteria. We speculate that inflammation or other mouse defenses may impact these bacteriophages," Kleiner said.

This is an image of the Mycobacterium smegmatis phage MicroWolf. / Credit: H. Hendricks, NC State Phage Hunters, E. Miller, NC State University.

Inflammation, the authors suggested, changes the levels of phages when IBD is occurring. It may be that phages kill off beneficial bacteria, and leaves only harmful bacteria in its place.

After looking at existing reports on the patterns in humans, they found there were many patterns that overlapped with those seen in mice.
 
"This was unexpected, as mouse studies are frequently viewed as limited when looking for links to human health, but we could show that there are consistent patterns in the viral community in inflammation in both mice and humans," Kleiner said.

"What we see in mice is consistent with what we see in humans with IBD," Duerkop said.

This work could help develop therapeutics using probiotics or some other thing that manipulates bacteria levels. "We could promote the growth of good bacteria - a kind of phage therapy," Duerkop suggested. "We could perhaps use phages as markers to identify someone predisposed to developing these diseases. While there is clearly more research to do, the potential is very exciting."

 

Sources: AAAS/Eurekalert!, North Carolina State University, University of Colorado Anschutz Medical Campus, Mayo Clinic, Nature Microbiology

About the Author
  • Experienced research scientist and technical expert with authorships on 28 peer-reviewed publications, traveler to over 60 countries, published photographer and internationally-exhibited painter, volunteer trained in disaster-response, CPR and DV counseling.
You May Also Like
APR 16, 2020
Health & Medicine
APR 16, 2020
Structural Basis of Receptor Recognition by SARS-CoV-2
As mortality and infection rates rise globally, it appears that SARS-CoV-2, the virus responsible for the COVID-19 pande ...
APR 29, 2020
Microbiology
APR 29, 2020
Bacteria Exposed to Antibiotics are 'Primed' to Gain More Resistance
Antibiotics are critical drugs that have saved millions of lives, but bacteria can also gain resistance to them, renderi ...
MAY 04, 2020
Microbiology
MAY 04, 2020
3D Print a Customizable Microscope for as Little as $18
Researchers have developed an open-source and customizable microscope that can be 3D-printed for only $18.
JUN 15, 2020
Microbiology
JUN 15, 2020
Research Shows Wearing a Face Mask Reduces the Risk of COVID-19 Infection
We've known that face masks can reduce the likelihood that asymptomatic people will spread the illness. But they are als ...
JUN 24, 2020
Neuroscience
JUN 24, 2020
Prenatal Stress May Influence Infant Gut Bacteria
Although prenatal stress has previously been associated with infant growth and development, exactly how they are linked ...
JUN 24, 2020
Microbiology
JUN 24, 2020
Genetic Variations Can Affect the Gut Microbiome
The small variations in the human genome aren't the only thing that make us unique. We also each carry communities of mi ...
Loading Comments...