Precise protein quantification is a crucial step in workflows involving protein extraction, isolation, and analysis. Accurate knowledge of the concentration of your protein sample is essential for proper setup and interpretation of experiments. It is also fundamental in understanding molecular interactions, signaling pathways, and pathological mechanisms, and in biomarker and drug discovery.

Learning Objectives: 

1. Describe how spectrophotometry, total protein assays, ELISA, Western blotting, and mass spectrometry are used for protein quantification.

2. Understand which options are best for different scenarios based on your research objectives, sample compatibility, sensitivity, dynamic range, and ease of use.

3. Recognize common challenges exist for each method such as interference from contaminants or addressing sample complexity.

As the fields of flow cytometry and spatial biology advance, researchers are uncovering exciting applications for the expanding data from their samples. Innovative reagents, fluidic and optical engineering, and advanced detection technologies significantly boost data speed, accuracy, and depth. Challenges persist, particularly in analyzing rare, clog-prone samples and deconvoluting fluorophore signals. This seminar will showcase data from two new spectral platforms and offer tips for maximizing their capabilities in advanced immunology and oncology research.

The spectral-enabled Invitrogen™ Attune™ Xenith™ Flow Cytometer, with acoustic focusing technology, advanced fluidics sensing, and UV to NIR detection helps provide deeper insights into even highly concentrated, high aggregate samples. Its software facilitates switching between conventional compensation and spectral unmixing, offering spillover guidance for each workflow. The Invitrogen™ EVOS™ S1000 Spatial Imaging System streamlines whole slide tissue imaging, enabling rapid 9-plex detection by means of integrated spectral unmixing.

We will address challenges in difficult sample types and offer solutions for overcoming common hurdles, with seminar outcomes including:

Understanding the impact of acoustic focusing on flow cytometric analysis.

Discovering how clog-resistant design maintains workflow efficiency and prevents poor-quality acquisitions.

Gaining insights into spectral workflows, maximizing panel complexity and acquisition efficiency with reduced-spillover fluorophores and optimized sample preparation.

Who should Attend:

Immunologists working on cell sorting, immune profiling, and flow cytometry

Oncologists focusing on cancer research and tumor immunology

Neuroscientists advancing brain spatial analysis and multiplex imaging techniques

Core lab researchers looking to enhance their workflows with advanced technologies

Anyone interested in pushing the boundaries of cellular analysis with spectral capabilities

Explore the advanced features of the Luminex® xMAP® INTELLIFLEX™ DR-SE instrument and the Invitrogen™ ProcartaPlex™ Signaling assays, with the novel dual reporter (DR) assay format. The talk will specifically focus on the innovative dual reporter capabilities to double your multiplex data and help revolutionize your way to investigate total and phosphorylated proteins and understand cellular signaling pathways.

The novel ProcartaPlex Signaling assay from Thermo Fisher Scientific enables you to quantitate and multiplex up to 16 parameters (8x phosphorylated and 8x total proteins) simultaneoulsy and requires only 4 hours from sample to results.

During the presentation, three key features of ProcartaPlex Signaling assays will be discussed:

- ProcartaPlex Signaling assays enable the detection of multiple phosphorylated and total proteins in a single well.

- ProcartaPlex Signaling assays offer the ability to obtain comparable results to ELISA and western blot analysis.

- ProcartaPlex Signaling assays enable the detection of low-abundance proteins and signaling molecules.

By attending this webinar, you will gain valuable insights into the capabilities of the xMAP INTELLIFLEX DR-SE instrument and ProcartaPlex Signaling assays. This webinar is beneficial for scientists interested in advancing their research through the utilization of cutting-edge technology and innovative assays.

Learning Objectives: 

1. Identify the key capabilities and features of the Luminex xMAP INTELLIFLEX DR-SE instrument and the dual reporter application.

2. Introduce the usability of the novel ProcartaPlex Akt Signaling Pathway assays for simultaneous detection of phosphorylated and total proteins.

3. Gain confidence in this novel technology through side-by-side comparison of ProcartaPlex Signaling assays with ELISA and western blot.

Millions lose the fight against cancer each year, but many cancers can be cured if detected early and treated effectively. Traditional tissue biopsies, while informative, are invasive and offer limited snapshots. Liquid biopsies offer a minimally invasive option to complement tissue biopsies, enabling the discovery of tumor derived circulating biomarkers that can be used for monitoring tumor progression.

At Thermo Fisher Scientific, we are committed to developing highly sensitive liquid biopsy-based assays and providing robust upstream sample preparation solutions. Among them, Dynabeads magnetic beads represent an ideal tool for the enrichment, isolation/release, and detection of various biomarkers from liquid biopsy samples. In this webinar, we will specifically address the key methods for enrichment of circulating tumor cells (CTCs), exosomes, and ctDNA biomarkers during the sample preparation process, which is a crucial step in the overall liquid biopsy-based workflow.

Learning Objectives: 

1. Learn about highly specific and sensitive CTC isolation and exosome enrichment methods for accurate cancer detection and monitoring. 

2. Gain insight into the target enrichment for library preparations for next generation sequencing workflows.

3. Discover innovative solutions for efficient isolation of circulating tumor cells (CTCs), extracellular vesicles, and circulating tumor DNA (ctDNA). 

In recent years, targeted next generation sequencing techniques have rapidly evolved enabling researchers to leverage less invasive liquid biopsy methods to accurately assess tumor heterogeneity and predict treatment outcomes. Analysis of circulating tumor DNA (ctDNA) shed into the blood stream by tumor cells can offer a comprehensive view into the genetic makeup of tumors, whereas traditional tissue biopsies are limited or incomplete.

Building on cell-free DNA (cfDNA) research toward using noninvasive methods in cancer management, Dr. Karen Page will share her experience in precision oncology research and the value, applications, and approaches for achieving the complete picture of tumor heterogeneity in breast cancer.

At Leicester, Dr. Page leads the Shaw laboratory's next-generation sequencing applications where she primarily focuses on liquid biopsy with automation enablement and subsequent molecular analysis for monitoring patients. During the webinar, she will cover the design and scale of a pilot study executed between the core lab and collaborating centers and speak to sample and workflow considerations. Demonstration of liquid biopsies used to monitor clonal evolution and response to therapy through mutations and copy number changes in cell free DNA (cfDNA) will be discussed and outlined along with approaches to enable detection of somatic variants and copy number alterations in CTCs.

Learning Objectives: 

1. Discover the value, applications, and approaches of using of circulating tumor DNA (ctDNA) for liquid biopsy research.

2. Explore workflow automation for blood processing and isolation of cell-free DNA (cfDNA), in addition to downstream cfDNA workflows.

3. Hear about approaches to enable detection of somatic variants, copy number alterations in circulating tumor cells (CTCs), and guided therapy.

This webinar delves into the fundamentals and applications of Polymerase Chain Reaction (PCR). We will begin with an introduction to the basic principles of PCR, discussing the essential components and the critical features of DNA polymerases that influence the success of the amplification process. The session will explore various PCR applications, providing guidance on selecting the appropriate enzymes for different purposes and highlighting recent technological improvements in the field.

Learning Objectives:

1. Introduction to PCR, including Basic principles and components, PCR product analysis, and important features of DNA polymerases.

2. Overview of PCR applications, including how to choose PCR enzymes based on common applications and additional PCR considerations.

Join our comprehensive 3D cell culture crash course webinar to gain a foundational understanding of this innovative technology. Unlike 2D methods, 3D cell culture better mimics the in vivo environment, providing more accurate models for studying cell behavior, disease mechanisms, and drug responses.

By the end, participants will be equipped to integrate 3D cell culture techniques into their research, leading to more relevant and innovative studies. Don't miss this opportunity to enhance your research skills!

1. What are 3D cell models?

2. What do I need to create a 3D cell model?

3. How do I make a 3D cell model?

4. How do I characterize my model?

5. How do I analyze my model?

At Thermo Fisher Scientific, our goal is to bring innovative new products and services that enable the development of more physiologically relevant disease models to help accelerate the drug discovery process. This seminar will highlight innovations in stem cell workflow, including suspension culture, PSC characterization, and the downstream differentiation needed to generate 3D stem cell-based disease models. In addition, specific examples will be provided of how stem cell researchers can leverage novel protocols and reagents to build relevant 3-dimensional neural, cardia, and hemopoietic-derived organoid models.