MAY 18, 2017 08:00 AM PDT

WEBINAR: Create Your Own Cellular Compound-Target Engagement Assay

SPONSORED BY: DiscoverX
C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Sr. Product Manager, DiscoverX Corporation
    Biography
      Dana Haley-Vicente, Ph.D., is currently a Sr. Product Manager at DiscoverX (Fremont, CA) managing cell-based assays, kits, and consumable products for drug discovery target discovery, screening, lead optimization, and SAR campaigns. She has been working in the life science drug discovery industry for over 16 years and received her Ph.D. in Pharmacology from University of California, Los Angeles.

    Abstract:

    DATE: May 18, 2017
    TIME: 8:00AM PDT, 11:00AM ET

    It is well established that ligand binding can protect proteins from thermal denaturation in a cellular milieu; however, the broad application of this cellular target engagement concept to drug discovery has been hindered by a lack of simple platforms with sensitive quantitative readouts. Cell-based thermal stabilization assays are valuable methods for particular applications, but they can require target-specific antibodies for immuno-assay readouts, can be low-throughput for Western blot formats, and can be biased against multi-domain proteins. 
    Here we will describe InCELL Pulse™, which uses DiscoverX's Enzyme Fragment Complementation (EFC) technology to overcome these limitations of current thermal stabilization assays. This unique platform allows you to rapidly measure quantitative cellular target engagement potency values for inhibitors of diverse protein classes, including kinases, methyltransferases, and hydrolases. The tools and workflow for designing your own InCELL Pulse assays for your favorite target will be described and validation data will be highlighted.

    Learning Objectives:

    • Overview of compound-target engagement
    • Tools and workflow to create your own cell-based target engagement assay
    • Applications of InCELL Pulse assays

    Show Resources
    You May Also Like
    AUG 27, 2019 09:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 09:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    OCT 02, 2019 11:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    JUN 19, 2019 10:00 AM PDT
    JUN 19, 2019 10:00 AM PDT
    DATE: June 19, 2019TIME: 10:00am PDT, 1:00pm EDT As we develop new methods to create more biologically relevant models for research in understanding disease etiology and in...
    JUN 26, 2019 09:00 AM PDT
    C.E. CREDITS
    JUN 26, 2019 09:00 AM PDT
    DATE: June 26, 2019TIME: 9:00am PDT, 12:00pm EDT An excessive number of software solutions are available to help manage your clinical, biobank, or biorepository sample inform...
    MAY 16, 2019 04:00 PM CEST
    C.E. CREDITS
    MAY 16, 2019 04:00 PM CEST
    DATE: May 16, 2019TIME: 7:00am PDT, 10:00am EDT, 4:00pm CEST The emergence of NGS is revolutionizing the microbiological sciences and transforming medicine. Deep sequencing has...
    JUN 05, 2019 05:00 PM CEST
    C.E. CREDITS
    JUN 05, 2019 05:00 PM CEST
    DATE: June 5, 2019TIME: 8:00am PDT, 11:00am EDT, 5:00pm CEST Eukaryotic cell cultures respond to the most subtle influence. Apart from the risk of contamination, minimal chan...
    Loading Comments...
    Show Resources