FEB 26, 2020 9:00 AM PST

The use of 3D Models in Hepatic Biology

Speakers
  • R & D Scientist, Cell Biology, Thermo Fisher Scientific
    Biography
      A native of Maryland, Julia is a graduate of The University of Maryland College Park, and Mount St. Mary's University where she received a B.S. in microbiology, and an MBA respectively.

      While pursuing her B.S at UMD, Julia launched her career as a research scientist at the National Institutes of Health, National Cancer Institute. After graduating she moved to Baltimore MD and continued to develop her career through conducting cancer research and gaining authorship on multiple publications at The Johns Hopkins University School of Medicine. Following her desire to establish a career in the biotechnology industry Julia joined the Advanced Cell Systems/ADME Tox team at Thermo Fisher Scientific. In her 4 years with the company she has utilized her extensive technical expertise as well as product knowledge and has worked in manufacturing (cell isolations), product management, and is currently an R&D Scientist in Cell Biology.

      Currently living in Frederick MD, in her free time Julia enjoys being outdoors, and cooking, as well as spending quality time with her baby daughter.
    • Director and Group Leader, Thermo Fisher Scientific
      Biography
        Dr. Kuninger leads research, development and commercialization of media systems for pluripotent stem cell culture & differentiation, neurobiology, and (non-hepatic) primary cell biology at Thermo Fisher Scientific in the Cell Biology business based in Frederick MD. His teams support numerous portfolios and have launched over 25 new products spanning stem cell culture & cryopreservation, differentiation (endo-, ecto- and meso-dermal lineages) and neurobiology over the past 3 years. David is a seasoned scientist and manager, experienced in media formulation & optimization, assay design and implementation, and troubleshooting. Expertise in GLP/GMP compliance, tech transfer and scale up, as well as verification and validation processes. Prior to starting at Thermo Fisher Scientific (legacy Invitrogen) in 2007 as Staff Scientist, he joined Oregon Health Sciences University as a Postdoctoral Fellow investigating the actions of insulin-like growth factors in the lab of Dr. Peter Rotwein, subsequently joining the faculty in the Department of Biochemistry at OHSU as a Research Instructor. He completed is PhD in Biochemistry and Genetics University of Texas Medical Branch in the laboratory of Dr. John Papaconstatinou and has a B.S. in Chemistry from the University of Oregon.

      Abstract
      DATE: February 26, 2020
      TIME: 9:00am PST
       
      3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications, as traditional 2D cell cultures lack the organizational complexity and longevity needed to serve as effective models.
       
      3D cell cultures allow for more physiologically realistic testing models, and as research techniques and applications are refined and optimized, the use of 3D cell culture, organoids, and spheroids in research areas such as disease modeling and regenerative medicine are becoming more useful.
       
      In this webinar, we will present data on different approaches to 3D hepatic biology research and how to transition into 3D using different liver cell/tissue types.
       
      We will cover the use of HepaRG, Kupffer, and co-cultures as well as primary hepatocytes in 3D modeling and present data on:
       
      1.  The creation of hepatic spheroids from primary hepatocytes and how to measure
      • Gene Expression
      • Basic CYP activity
      • Drug dosing
      • and more
      2. Use of HepaRG in 3D modeling for quicker culture formations, consistent modeling, and reproducible results.
       
      3. Forming animal hepatic spheroids and the advantages of using 3D spheroids over 2D cultures. 
       
       
      Webinars will be available for unlimited on-demand viewing after live event.
       
      LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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      The use of 3D Models in Hepatic Biology


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