SEP 27, 2018 8:00 AM PDT

Achieving higher throughput with multiplexed phage display

Sponsored by: ForteBio LLC
Speaker
  • Software Product Manager, Molecular Devices
    Biography
      Dr. Thomas Hengstl is currently a Product Manager for Software and Compliance with Molecular Devices. Prior to joining the company, he received his PhD in Biophysics and Biochemistry through a joint international, industrial program between the University of Natural Sciences Salzburg and Genentech Inc. The development of the multiplexed phage display workflow described in the webinar was a product of his PhD program.

    Abstract

    DATE: September 27, 2018
    TIME: 8:00AM PDT

    Recent advances in phage display for molecular evolution incorporate deep sequencing to enable greater library coverage. However, severe gaps and limitations associated with next generation sequencing (NGS) library construction and biophysical follow-up of potential hits remain.

    Multiplexed phage display (MPD, Figure 1) provides a simple, robust, and seamless high-throughput-ready version of phage display that can simultaneously screen and bioinformatically analyze up to 96 peptide phage libraries in parallel. As a case study, raising peptide binders against the low-density lipoprotein receptor-related protein 6 (LRP6) using MPD generated more detailed results in a shorter time then standard phage display methods. To accommodate the large number of potential lead peptides from the screening, the ForteBio Octet system was used to characterize streptavidin peptide fusions generated by in vitro translation.

    Figure 1| Graphical abstract of the workflow of MPD|

    After the plate-based discovery (selection) process the DNA is extracted from the viral particles and libraries for NGS are generated. Based on the sequencing results from the target and control phage libraries, several peptides are selected for the biophysical characterization. Those peptides are then expressed as Streptavidin fusions and binding kinetics are measured using biolayer interferometry (BLI).

     

    Learning Objectives:

    • How to use next generation sequencing for phage display
    • How to generate streptavidin peptide fusions quickly and inexpensively

    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    MAR 16, 2021 10:00 AM PDT
    C.E. CREDITS
    MAR 16, 2021 10:00 AM PDT
    Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    DEC 17, 2020 9:00 AM PST
    C.E. CREDITS
    DEC 17, 2020 9:00 AM PST
    Date: December 17, 2020 Time: 9:00am (PST), 12:00pm (EST) Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging moda...
    SEP 27, 2018 8:00 AM PDT

    Achieving higher throughput with multiplexed phage display

    Sponsored by: ForteBio LLC

    Specialty

    Biotechnology

    Antibodies

    Biochemistry

    Cancer Research

    Molecular Biology

    Immunology

    Cell Biology

    Assay Development

    Cancer Therapeutics

    Cell Culture

    Drug Discovery

    Biomarkers

    Cancer Diagnostics

    Infectious Disease

    Genetics

    Geography

    North America64%

    Asia15%

    Europe13%

    Africa3%

    South America2%

    Oceania2%

    Registration Source

    Website Visitors83%

    Email Promotions7%

    WAVE Newsletter Promotion7%

    Social Media Promotions2%

    Website Banners1%

    Job Title

    Scientist23%

    Research Scientist15%

    Executive12%

    Student12%

    Educator/Faculty8%

    Facility/Department Manager7%

    Medical Laboratory Technician6%

    Medical Doctor/Specialist5%

    Post Doc4%

    Clinical Laboratory Scientist3%

    Lab Management3%

    Marketing/Sales2%

    Engineer1%

    Organization

    Biotech Company22%

    Academic Institution16%

    Research Institute9%

    Pharmaceutical Company8%

    Government6%

    Hospital6%

    Non-Profit Organization5%

    Life Science Company5%

    Clinical Laboratory5%

    Medical School3%

    Industrial Company3%

    Medical Center2%

    Consultant1%

    Contract Research Organization (Cro)1%

    Other5%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more