APR 16, 2015 08:00 AM PDT

Advances in Sedimentation Analysis

Speakers
  • Director, Bioinformatics Core Facility, University of Texas Health Science Center
    Biography
      Borries Demeler started his education at the University of Montana, earning a degree in chemistry and mathematics. He then joined Prof. Ken van Holde at Oregon State University for his graduate studies, earning a PhD in biochemistry and biophysics with his work involving biophysical characterization of chromatin. It was in Dr. van Holde's laboratory that he first learned about AUC on a 30 year old Beckman Model-E instrument with UV absorbance optics and a strip chart recorder. He digitized the instrument and wrote his first version of UltraScan to analyze AUC experiments. Next, he moved to Texas to join Prof. Jeff Hansen's laboratory at the University of Texas Health Science Center in San Antonio for a postdoctoral position, where he ported UltraScan to the XLA/I instruments. After completing his postdoc, he joined the Biochemistry faculty at UTHSCSA and became director for the Center for Analytical Ultracentrifugation of Macromolecular Assemblies (CAUMA), which he still manages today. CAUMA is a premier service laboratory in the US open to any researcher investigating solution behavior of macromolecules, polymers, nanoparticles and assemblies. Dr. Demeler continued his development of AUC analysis software, securing multi-million $ funding through NIH and NSF grants to create the most advanced AUC analysis software available today, called UltraScan. Dr. Demeler was the first to employ high-performance computing to maximize resolution, information content and increase throughput for AUC analysis. He has authored over 130 manuscripts and book chapters, about half of them on methods development for AUC. Dr. Demeler currently co-develops the XSEDE Science Gateway, a $5 Mio. NSF grant with Indiana University and San Diego Supercomputing Center to advance access to high-performance computing for AUC and a a broad range of other science domains. He holds an annual AUC workshop in San Antonio where he teaches the foundation of hydrodynamics and mentors future AUC experts.

    Abstract:
    In recent years, sophisticated new methods for sedimentation data analysis have opened up new avenues for leveraging AUC technology in a wide range of technology sectors, including life science research, pharmaceutical QC, material, polymer and nano science. In this talk, Borries Demeler will highlight some of the approaches used in his lab to extract ever more information from sedimentation velocity experiments. In this talk he will address ways of accurately characterizing systems at high resolution that are heterogeneous in both size and shape, and present methods for measuring the strength of macromolecular interactions by fitting discrete binding models to whole boundary sedimentation velocity experiments.

    Learning Objectives:
    • Learn ways to accurately characterize systems at high resolution that are heterogeneous in both size and shape
    • Learn approaches used to extract ever more information from sedimentation velocity experiments
    • See methods for measuring the strength of macromolecular interactions by fitting discrete binding models to whole boundary sedimentation velocity experiments

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