APR 16, 2015 8:00 AM PDT

Advances in Sedimentation Analysis

Speakers
  • Director, Bioinformatics Core Facility, University of Texas Health Science Center
    Biography
      Borries Demeler started his education at the University of Montana, earning a degree in chemistry and mathematics. He then joined Prof. Ken van Holde at Oregon State University for his graduate studies, earning a PhD in biochemistry and biophysics with his work involving biophysical characterization of chromatin. It was in Dr. van Holde's laboratory that he first learned about AUC on a 30 year old Beckman Model-E instrument with UV absorbance optics and a strip chart recorder. He digitized the instrument and wrote his first version of UltraScan to analyze AUC experiments. Next, he moved to Texas to join Prof. Jeff Hansen's laboratory at the University of Texas Health Science Center in San Antonio for a postdoctoral position, where he ported UltraScan to the XLA/I instruments. After completing his postdoc, he joined the Biochemistry faculty at UTHSCSA and became director for the Center for Analytical Ultracentrifugation of Macromolecular Assemblies (CAUMA), which he still manages today. CAUMA is a premier service laboratory in the US open to any researcher investigating solution behavior of macromolecules, polymers, nanoparticles and assemblies. Dr. Demeler continued his development of AUC analysis software, securing multi-million $ funding through NIH and NSF grants to create the most advanced AUC analysis software available today, called UltraScan. Dr. Demeler was the first to employ high-performance computing to maximize resolution, information content and increase throughput for AUC analysis. He has authored over 130 manuscripts and book chapters, about half of them on methods development for AUC. Dr. Demeler currently co-develops the XSEDE Science Gateway, a $5 Mio. NSF grant with Indiana University and San Diego Supercomputing Center to advance access to high-performance computing for AUC and a a broad range of other science domains. He holds an annual AUC workshop in San Antonio where he teaches the foundation of hydrodynamics and mentors future AUC experts.

    Abstract:
    In recent years, sophisticated new methods for sedimentation data analysis have opened up new avenues for leveraging AUC technology in a wide range of technology sectors, including life science research, pharmaceutical QC, material, polymer and nano science. In this talk, Borries Demeler will highlight some of the approaches used in his lab to extract ever more information from sedimentation velocity experiments. In this talk he will address ways of accurately characterizing systems at high resolution that are heterogeneous in both size and shape, and present methods for measuring the strength of macromolecular interactions by fitting discrete binding models to whole boundary sedimentation velocity experiments.

    Learning Objectives:
    • Learn ways to accurately characterize systems at high resolution that are heterogeneous in both size and shape
    • Learn approaches used to extract ever more information from sedimentation velocity experiments
    • See methods for measuring the strength of macromolecular interactions by fitting discrete binding models to whole boundary sedimentation velocity experiments

    Show Resources
    You May Also Like
    OCT 02, 2019 11:00 AM PDT
    OCT 02, 2019 11:00 AM PDT
    DATE: October 2, 2019TIME: 11:00am PDT, 2:00pm EDT Ditch the Excel spreadsheets and manage your molecular workflows entirely in your LIMS Achieve configuration of molecular workf...
    SEP 05, 2019 4:00 PM CEST
    C.E. CREDITS
    SEP 05, 2019 4:00 PM CEST
    DATE: September 5, 2019TIME: 7:00am PT, 10:00am ET, 4:00pm CEST PCR (Polymerase Chain Reaction) has gone through a massive evolution since its development in 1983. Besides it...
    FEB 26, 2020 9:00 AM PST
    C.E. CREDITS
    FEB 26, 2020 9:00 AM PST
    DATE: February 26, 2020 TIME: 9:00am PST 3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications as traditional...
    AUG 27, 2019 9:00 AM PDT
    C.E. CREDITS
    AUG 27, 2019 9:00 AM PDT
    DATE: August 27, 2019 TIME: 9:00am PDT, 12:00pm EDT Immunotherapies targeting PD-1 or PD-L1 have proven remarkably effective for treating cancer in some patients, with considerabl...
    JAN 23, 2020 9:00 AM PST
    C.E. CREDITS
    JAN 23, 2020 9:00 AM PST
    DATE: January 23, 2020 TIME: 9:00am PST, 12:00pm EST...
    OCT 29, 2019 7:00 AM PDT
    C.E. CREDITS
    OCT 29, 2019 7:00 AM PDT
    DATE: October 29, 2019TIME: 7:00am PDT3D cell culture systems have recently emerged as tools for reproducing the cellular environment and the organization of tissues/organs, where cel...
    Loading Comments...
    Show Resources