SEP 12, 2018 6:00 AM PDT

Advancing Metagenomic Tools for Monitoring Antibiotic Resistance and Pathogen Gene Markers in Water and the Environment

Speaker
  • Professor of Civil & Environmental Engineering, Associate Dean and Director of Interdisciplinary Graduate Education, Virginia Tech
    Biography
      Amy Pruden is the W. Thomas Rice Professor of Civil and Environmental Engineering at Virginia Tech. Her research focuses on bringing a microbial ecological perspective to understanding and advancing design and management of environmental systems. Pruden is a leading expert on water-based pathogens and antibiotic resistance. In 2012-2013, she led a Water Research Foundation expert workshop and report on Opportunistic Pathogens in Premise Plumbing: Epidemiology, Microbial Ecology, and Engineering Controls, in which a multi-stakeholder framework for public health protection was developed. In 2016, she co-organized a workshop sponsored by the Alfred P. Sloan Foundation at Emory University, From Watersheds to Showerheads: Improving Legionella Risk Management in the 21st Century. Her current research, funded by The Alfred P. Sloan Foundation, focuses on how engineering design shapes the composition of the microbiome of tap water and implications for control and spread of Legionella, Naegleria fowleri, and antibiotic resistance genes. She has authored over 120 peer-reviewed scientific journal articles and is currently PI on a USDA AFRI project aimed at developing computational tools for identifying critical control points for mitigating the spread of antibiotic resistance. Dr. Pruden is the recipient of the Presidential Early Career Award in Science and Engineering and the Paul L. Busch Award for innovation in water research. She holds a B.S. in biology and a Ph.D. in environmental science, both from the University of Cincinnati.

    Abstract

    Next-generation DNA sequencing has opened new possibilities for microbial water quality monitoring, particularly for addressing emerging concerns like antibiotic resistance and opportunistic pathogens.   Here we will demonstrate how the approach is being applied to survey antibiotic resistance genes and pathogen gene markers in various water and environmental systems, including drinking water, wastewater, reclaimed water, and agricultural manure management systems.  Current efforts are focused on deriving various metrics from metagenomic data for assessing presence of these targets and potential to spread undesirable traits, such as antibiotic resistance and virulence genes.  As DNA sequencing and bioinformatic technologies are rapidly developing, development and validation of various approaches is needed, along with adoption of standardized methods by relevant stakeholders.

    Learning Objectives: 

    1. Describe what shotgun metagenomic sequencing is and benefits for water analysis
    2. Describe current limitations to shotgun metagenomic approaches for pathogen and resistance monitoring
    3. Describe new developments and approaches for advancing metagenomic tools for water monitoring


    Show Resources
    You May Also Like
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    MAY 11, 2021 10:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
    DEC 02, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 02, 2020 8:00 AM PST
    DATE: December 2nd, 2020 TIME: 08:00am PDT, 11:00pm EDT Bioreactors and shakers are used to cultivate microorganisms, plant, insect, and mammalian cells in different volumes. Upscaling of pr...
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    C.E. CREDITS
    JUN 09, 2021 7:00 AM PDT
    Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
    Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
    MAR 16, 2021 10:00 AM PDT
    C.E. CREDITS
    MAR 16, 2021 10:00 AM PDT
    Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
    DEC 16, 2020 8:00 AM PST
    C.E. CREDITS
    DEC 16, 2020 8:00 AM PST
    Date: December 16, 2020 Time: 8:00am (PST), 11:00am (EST) Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better under...
    DEC 15, 2020 10:00 AM PST
    C.E. CREDITS
    DEC 15, 2020 10:00 AM PST
    DATE: December 15, 2020 TIME: 10:00am PST Scientists from Thermo Fisher Scientific will walk us through the world of microorganisms. They will discuss their most recent research on viruses,...
    SEP 12, 2018 6:00 AM PDT

    Advancing Metagenomic Tools for Monitoring Antibiotic Resistance and Pathogen Gene Markers in Water and the Environment


    Specialty

    Gene Sequencing

    Dna Sequencing

    Molecular Biology

    Clinical Research

    Animal Sciences

    Pcr/rt-Pcr/real-Time Pcr

    Dna

    Molecular Diagnostics

    Genetics

    Gene Expression

    Biotechnology

    Bioinformatics

    Microbiology

    Genomics

    Agricultural Sciences

    Geography

    Asia60%

    North America20%

    South America20%

    Registration Source

    Website Visitors100%

    Job Title

    Educator/Faculty40%

    Research Scientist20%

    Medical Laboratory Technician20%

    Chemist20%

    Organization

    Academic Institution40%

    Government/public20%

    Manufacturer - Other20%


    Show Resources
    Loading Comments...
    Show Resources
    Attendees
    • See more