JUN 08, 2017 10:00 AM PDT

WEBINAR: Analysis of the transcriptome of carriers of pathological variants in PSEN1, PSEN2 and APP that cause Alzheimer's Disease

Sponsored by: Lexogen
Speaker

Abstract

DATE: June 8, 2017
TIME: 10:00AM PDT, 1:00PM EDT, 7:00PM CEST

Alzheimer’s Disease (AD) is the result of complex interactions between risk factors that cause pleiotropic changes in molecular networks linking a host of biological processes. A variety of genetic factors have been shown to contribute to risk with varying degrees of penetrance: the identification of mutations in the amyloid-beta precursor protein (APP), presenilin (PSEN1 and PSEN2) genes that cause Mendelian forms of AD represented key milestones for understanding the initial mechanisms and pathways involved in AD pathogenesis. Remarkably, variants in these genes confer a different transcriptomic profiles, and mutation carriers clustered separately from their non-carrier siblings. New evidences provide support for both neuronal and glial specific pathways contributing to pathogenesis. However, little is understood about how the genetic loci and molecular changes are organized into common networks. We combined transcriptomic cell-type profiling and network co-expression analyses to study a unique collection of human postmortem brain tissue ascertained to represent the AD Mendelian mutations.

Using novel digital deconvolution approaches, we derived cell-type specific expression. We ascertain the distribution of neuros, microglia, oligodendrocytes and astrocytes in a collection of more than 1500 AD and non-demented subjects. We derived gene regulatory networks employing the expression corrected for the distinct cell-type distributions, and identified modules that cluster genes that harbor variants usually associated with both early-onset autosomal dominant (PSEN1) and late-onset sporadic classifications of AD (SOD1, BACE1, PICALM, SLC4A2).

Understanding variant-specific effects is of an immense importance for the elucidation of the underlying biology of the Alzheimer Disease. Our initial analysis reveals a transcriptional regulation module that link that early-onset autosomal dominant and late-onset sporadic genes.

Learning Objectives:

  • Identify confounding factors that can affect transcriptomic analyses and learn how to address them
  • Familiarize with machine learning techniques that allow to validate results when analyzing dataset with a reduced number of samples
  • Learn digital deconvolution approaches to infer cell composition from RNA-seq data
  • Learn how transcriptomic profiles can reveal gene co-expression networks

 


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
JUL 15, 2021 9:00 AM PDT
JUL 15, 2021 9:00 AM PDT
Date: July 15, 2021 Time: 9:00am (PDT), 12:00pm (EDT) The Pisces workflow robust, easy-to-use, end-to-end multi-omics solution for highly multiplexed targeted Spatial RNA analysis. VeranomeB...
SEP 17, 2021 12:00 PM CST
C.E. CREDITS
SEP 17, 2021 12:00 PM CST
Date: September 16, 2021 Time: 9:00pm (PDT), 12:00am (EDT) 3D cellular models like organoids and spheroids offer an opportunity to better understand complex biology in a physiologically rele...
OCT 27, 2021 6:00 AM PDT
C.E. CREDITS
OCT 27, 2021 6:00 AM PDT
Date: October 27, 2021 Time: 6:00 AM PDT, 9:00 AM EDT Etanercept is a recombinant Fc fusion protein therapeutic that has a complex distribution of post-translation modifications (PTM), such...
JUN 08, 2017 10:00 AM PDT

WEBINAR: Analysis of the transcriptome of carriers of pathological variants in PSEN1, PSEN2 and APP that cause Alzheimer's Disease

Sponsored by: Lexogen

Specialty

Molecular Biology

Genetics

Cancer Research

Genomics

Molecular Diagnostics

Immunology

Cell Biology

Biotechnology

Laboratory Testing

Biochemistry

Gene Expression

Bioinformatics

Infectious Disease

Clinical Diagnostics

Neuroscience

Geography

North America63%

Europe17%

Asia10%

South America5%

Africa2%

Registration Source

Website Visitors100%

Job Title

Research Scientist15%

Student13%

Executive10%

Educator/Faculty9%

Post Doc9%

Scientist9%

Facility/Department Manager8%

Medical Laboratory Technician7%

Medical Doctor/Specialist5%

Clinical Laboratory Scientist5%

Lab Management5%

Marketing/Sales1%

Genetic Counselor1%

Consultant1%

Chemist1%

Organization

Academic Institution13%

Clinical Laboratory8%

Hospital6%

Biotech Company5%

Research Institute5%

Life Science Company3%

Medical School3%

Medical Center3%

Government2%

Pharmaceutical Company2%

Ambulatory Care2%

Contract Research Organization (Cro)1%

Non-Profit Organization1%

Medical Device Company1%

Other3%


Show Resources
Loading Comments...
Show Resources