Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders
Reproducibility and antibody validation standards are two significant challenges facing scientific researchers today. To address these issues, Thermo Fisher Scientific—a leading producer of antibodies— organized and supported a group of leading researchers from several global institutions. This International Working Group for Antibody Validation (IWGAV) brainstormed and developed a set of proposed standards to optimize antibody validation methods with a specific emphasis on antibody specificity verification. As a follow up, in fall 2016, the Global Biological Standards Institute (GBSI) hosted a meeting attended by several IWGAV members and representatives from antibody producers, pharmaceutical companies and leading academic research labs. Attendees reviewed and validated the IWGAV proposed standards and discussed strategies designed to implement these standards across the industry.
In this event, Thermo Fisher Scientific will host a live, virtual round table discussion in which representative IWGAV members and others from the research community will participate in a dialogue about antibody validation standards and practices, with the goal of finding consensus for how to further methods for verifying antibodies used for common laboratory techniques—such as western blotting, ELISA, immunohistochemistry, immunofluorescence. The panel will also discuss how these new standards may be adapted to validate antibodies intended for flow cytometry.
Antibody validation with immunoprecipitation/mass spectrometry and orthogonal methods
Speaker: John Rogers, PhD, Thermo Fisher Scientific
Super Bright antibody conjugates for flow cytometry—Bright polymer dyes for the violet laser
Speaker: Castle Funatake, PhD, Thermo Fisher Scientific
Chemiluminescent western blot detection: Bright and bold detection no matter how scarce your target
Speaker: Emily Halbrader, Thermo Fisher Scientific
The new generation in immunoassays
Speaker: David Bourdon, PhD, Thermo Fisher Scientific