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JUN 17, 2020 10:30 AM PDT

Applications of Flow Cytometry and Imaging Flow Cytometry to COVID-19 Research

Sponsored by: LUMINEX
C.E. Credits: P.A.C.E. CE Florida CE
Speakers
  • Manager, Senior Scientist
    Biography
      Haley R. Pugsley, Ph.D., is a Manager, Senior Scientist for the Amnis Flow Cytometry products at Luminex Corporation. Haley received her Ph.D. in Analytical Chemistry from the University of Washington working under the direction of Professor Norman J. Dovichi. At the University of Washington she used capillary electrophoresis to study protein fingerprinting of breast cancer tissue and created two reporter cell lines to investigate the expression of RecA in Deinococcus radiodurans. Dr. Pugsley did her postdoctoral fellowship at Hematologics, Inc. under the direction of Dr. Michael R. Loken where she focused on clinical flow cytometry and its use in diagnosing hematologic malignancies. Dr. Pugsley joined Amnis (now part of Luminex Corporation) in 2011 developing applications for the ImageStreamX Mk II, FlowSight and CellStream flow cytometers. During her time at Luminex Corporation, Dr. Pugsley has worked on many applications including autophagy, immunological synapse, algae biomass, cell signaling, and most recently extracellular vesicles.
    • Director, Flow Cytometry Assays & Application
      Biography
        Dr. Kamala Tyagarajan is Director, Flow Cytometry Assays and Applications at Luminex Corporation where her team is responsible for applications and assay development on Luminex's microcapillary based flow cytometry platforms. Dr. Tyagarajan was previously R&D Director, MilliporeSigma, EMD Millipore and Guava Technologies and has brought out a range of flow cytometry products for cellular health, immunology and blood-based diagnostics. Previously, Dr. Tyagarajan worked at Lynx Therapeutics (now Illumina) developing novel fluorescence- based and mass spectrometry based proteomic technologies. Kamala obtained her PhD Degree at University of California, Berkeley and postdoctoral research at UC San Francisco/UC Berkeley working on parietal cell glycobiology and proteomics.

      Abstract

      COVID 19 has resulted in diverse research needs ranging from mechanism and impact of the disease, to solutions to identify, combat or treat the disease.  Cellular technologies such as flow and imaging cytometry can provide powerful information in this discovery process. In this webinar we will discuss cellular technologies and solutions available from Luminex Corporation to aid COVID 19 research.  Our Guava® Muse® and Guava® easyCyte™ platforms leverage the power of microcapillary cytometry and small sample sizes to provide quick, rapid methods for cellular counts and viability as well as characterization of immune cells providing information such as  absolute CD4 T , CD8 T cell, B cells and total lymphocytes with ease.  The webinar will also discuss cell health assays and capabilities on the platforms that allow for quick assessment of apoptosis and cytotoxicity from compound treatments especially in drug repurposing studies.

      Our imaging cytometry platform, the ImageStream®X Mk II combines the quantitative power of flow cytometry with the spatial information provided by microscopy into one system. The ImageStream classifies cell images not only based on fluorescence intensity but the relative distribution of that intensity as well as morphology. The platform provides the capability to study signaling cascades and translocation of transcription factors, which may be important in immune cell activation and host defense against pathogens. It also provides the capability to study white blood cell sub types of interest using morphologic and fluorescence parameters.  The ImageStream® can also measure individual fluorescently labeled viruses; the new High Gain mode for the ImageStream®X Mk II increases the signal obtained from these small viruses while minimally increasing the noise. In this presentation 3 applications will be discussed for the ImageStream®, individual murine leukemia virus-sfGFP reference particles demonstrating High Gain mode, shape change, and nuclear translocation.

      Learning Objectives:

      1. Important questions  of COVID 19 research that can be tackled using flow cytometry

      2. Instrument and assay tools available on Muse and Guava platforms for COVID 19 research

      3. Imaging flow cytometry enables applications that can’t be performed by flow cytometry or microscopy alone.


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