Consistent accuracy in reporting quantitative viral load results is critically important to patients, their physicians and to the clinical laboratory. In clinical practice, quantitative viral load results are used both to diagnose the severity of disease and are crucial for guiding patient management and treatment. Such clinical decision-making relies on the accuracy of clinical laboratory testing. This seminar will provide an overview of quantitative viral load testing and how run controls, when used as part of an overall laboratory quality management system, can help ensure accurate results are reported. Data from HIV-1, HBV and HCV qPCR assays and QC Sigma metrics will be presented.