MAY 19, 2022 8:00 AM PDT

Automated sample preparation for reproducible clinical proteomics

Sponsored by: Covaris, Inc.
Speakers

Abstract
Date: May 19, 2022
Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST)
 
Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classification and diagnostics, however their standardized use is still prevented by several bottlenecks in sample handling. Here, I will present how we integrated AFA-based ultrasonication in an automated proteomic workflow to enable routine, robust and comprehensive proteome analysis with minimal hands-on time. This can be applied to cells, fresh tissue and FFPE tissue, thus spanning the full range of clinical sample types and indicating universal utility. Moreover, 96 samples can be processed simultaneously, ensuring throughput under standardized conditions. Sensitivity of the approach will be shown from low-input experiments starting from 100-1000 cells. Data will be shown of the implementation of this pipeline to a cohort of 96 ependymoma tumor samples, where we integrated the obtained proteomic data with other omics layers allowing individualized molecular profiling and disease sub-classification.
 
Benefits of Adaptive Focused Acoustics for Proteomics Lab
 
Proteomics requires strategies capable of processing hundreds of samples in a robust and reproducible manner, independently of the origin of the material of interest. In this webinar I will show how the Proteomics Facility of the Biomedical Center at the University of Munich takes advantage of AFA-based ultrasonication to establish universal workflows that can be applied to the proteomic studies of diverse samples including FFPE, thrombi or low number of cells. The standardization, reproducibility and throughput of these workflows facilitated the analysis of large cohorts and provided our collaboration partners with proteomics data ready for clinical or biological interpretation and integration with other omics information.
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

Show Resources
You May Also Like
MAY 17, 2022 9:00 AM PDT
MAY 17, 2022 9:00 AM PDT
Date: May 17, 2022 Time: 9:00am (PDT), 12:00pm (EDT), 8:00pm (CEST) Gene therapeutics have great potential to treat many severe diseases in an unprecedented, targeted manner. The biopharmace...
APR 28, 2022 8:00 AM PDT
APR 28, 2022 8:00 AM PDT
Date: April 28, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CEST) Human pluripotent stem cells (PSCs) and their derivatives hold great potentials in...
MAR 23, 2022 11:00 AM PDT
MAR 23, 2022 11:00 AM PDT
Date: March 23, 2021 Time: 11:00am (PDT), 2:00pm (EDT), 8:00pm (CEDT) In this presentation, Dr. Middleton will review the development and deployment of large-scale saliva-based COVID-19 test...
MAR 16, 2022 8:00 AM PDT
C.E. CREDITS
MAR 16, 2022 8:00 AM PDT
Date: March 16, 2022 Time: 8:00am (PDT), 11:00am (EDT), 5:00pm (CET) Handling of potent and/or hazardous substances is commonplace in sev.....
NOV 30, 2021 10:00 AM PST
C.E. CREDITS
NOV 30, 2021 10:00 AM PST
Date: November 30, 2021 Time: 10:00am (PDT), 1:00pm (EDT) The prevalence of thyroid disease worldwide has served as a catalyst for healthcare providers to study various tools and methods to...
DEC 09, 2021 11:00 AM PST
C.E. CREDITS
DEC 09, 2021 11:00 AM PST
Date: December 09, 2021 Time: 11:00am (PDT), 2:00pm (EDT) The burden of antimicrobial resistance (AMR) has been acknowledged worldwide by leading health institutes. Besides the need for new...
Loading Comments...
Show Resources