MAY 31, 2018 9:00 AM PDT

Automation of Live-Cell Metabolic Assays with the Seahorse XFe96 Analyzer and Bravo Automated Liquid Handling Platform

Presented at: Lab Automation 2018
Sponsored by: Agilent
Speakers
  • Product Manager, Agilent Technologies
    Biography
      Dr. Burroughs received her Ph.D. in Chemistry from Penn State University, focusing on protein biochemistry. She did a post-doc at the University of Pittsburgh School of Medicine, then joined startup Cellomics Inc. in 1998 at the beginning of the era of high content screening. At Cellomics, Sarah worked in R&D and product development as well as product management. After acquisitions by Fisher Scientific then Thermo, Sarah worked as a field applications scientist for HCS products. After 11 years with Cellomics, Sarah joined PerkinElmer as an applications scientist with the automated liquid handling group. In 2013 Sarah became a product manager at Seahorse Bioscience, managing projects involving both assays and instruments. Seahorse Bioscience was acquired in 2015 by Agilent Technologies, where Sarah continues as a product marketing manager for Seahorse XF instruments.
    • Product Specialist, Agilent Technologies
      Biography
        Dr. Evans received her Ph.D. in Pharmacology from The University of Texas Health Science Center at San Antonio focusing on Serotonin 1A receptor pharmacology. She did a post-doc at the University of Houston, College of Pharmacy and Pharmaceutical Sciences concentrating on cardiovascular and respiratory pharmacology. Kenda joined Encysive Pharmaceuticals in the high throughput screening lab where she worked through many screening campaigns and had specific project management responsibilities. After 5 years with Encysive Pharmaceuticals, she joined PerkinElmer as a field application scientist with the high throughput screening reagents and plate readers group. In 2011 Kenda joined Agilent Technologies as a product specialist in the automation workflow group where she continues to work to build out new applications using the Bravo liquid handler for automated sample preparation.

      Abstract

      Cell-based assays are more biologically relevant than biochemical assays yet significantly more scalable than in vivo experiments.  However, care must be taken in sample preparation to ensure high quality results, and liquid handling automation is a key component of a robust assay workflow.  The Agilent Seahorse XFe96 Analyzer is a label-free metabolic analysis platform that enables simultaneous measurement of mitochondrial function and glycolysis, enabling assessment of metabolic phenotype in live cells.  This workshop will illustrate the use of an Agilent Bravo Liquid Handling Platform and assorted Microplates to prepare samples for Seahorse analysis.
       

      Learning Objectives: 

      1. Understand how and in what stages automated liquid handling can aid sample prep for cell-based assays
      2. How energy production pathway knowledge contributes to understanding metabolic phenotype
      3. How metabolic phenotype can be used to detect mitochondrial toxins


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