MAR 29, 2018 1:30 PM PDT

Cannabidiol Treatment of Epilepsy and Autism Through Restoration of GABAergic Signaling

Speaker

Abstract

Cannabidiol (CBD) has emerged as a promising anti-epileptic drug in otherwise treatment-resistant genetic epileptic disorders. However, the clinical trials have been limited by their use of CBD only as an add-on therapy, their lack of assessment of CBD on comorbid phenotypes, and their lack of insight into CBD’s therapeutic mechanism. To address these limitations, we tested CBD’s effects on behavior and physiology in our genetic mouse model of Dravet Syndrome (DS). DS is a severe childhood epileptic disorder characterized by treatment resistant epilepsy, as well as cognitive and social deficits consistent with autism. Our DS mice recapitulate these core behavioral phenotypes and are thus a powerful precision medicine tool to test the efficacy of CBD as a monotherapy in DS. We find that acute CBD administration reduced the thermally-induced seizures that model febrile seizures in the early stage of DS. Twice-daily CBD administration also reduced the frequency of spontaneous seizures that characterize the later stages of DS.

These anti-epileptic effects were associated with an enhancement in GABAergic signaling in the dentate gyrus of the hippocampus, which is mediated by CBD antagonism of GPR55 signaling. Furthermore, we find that CBD rescued social interaction deficits and abnormal social responses in our DS mice. Together, our data suggest that CBD is an effective monotherapy against epileptic seizures and autism in DS. Our results also reveal that GPR55 signaling is an important target for CBD’s therapeutic benefits.

Learning Objectives:

  •  Be able to describe cannabidiol's benefits as a monotherapy in treating epilepsy and autism
  •  Understand the cellular mechanism by which cannabidiol rescues brain function that's disrupted   by genetic mutations
     

Show Resources
You May Also Like
FEB 15, 2023 7:00 AM PST
FEB 15, 2023 7:00 AM PST
Date: February 15, 2023 Time: 7:00am (PST), 10:00pm (EST), 4:00pm (CET) While not all microscopy samples can fluoresce, all can scatter light, and this scattered light can be imaged. This ha...
NOV 16, 2022 7:00 PM PST
C.E. CREDITS
NOV 16, 2022 7:00 PM PST
Date: November 16, 2022 Time: 2:00pm (AEST) Date: November 17, 2022 7:00pm (PST), 10:00pm (EST), 4:00am (CET) The growth in FDA-approved cell and gene therapy products for the treatment of d...
AUG 17, 2022 10:00 AM PDT
AUG 17, 2022 10:00 AM PDT
DATE: August 17, 2022 TIME: 10:00am PDT, 1:00pm EDT Detection and/or amplification of target DNA sequences by polymerase chain reaction (PCR) is a prevalent method in molecular biology. PCR...
AUG 24, 2022 7:00 AM PDT
AUG 24, 2022 7:00 AM PDT
Date: August 24, 2022 Time: 7:00am (PDT), 10:00pm (EDT), 4:00pm (CEST) Light field microscopy was first introduced in 2006, and allows users to capture the 4D light field within the microsco...
NOV 07, 2022 9:00 PM PST
C.E. CREDITS
NOV 07, 2022 9:00 PM PST
Date: November 8, 2022 Time: 1:00pm (SGT), 12:00pm (EST), 6:00am (CET) Antibodies are vital tools in many of the laboratory techniques that are used...
OCT 11, 2022 8:00 AM PDT
C.E. CREDITS
OCT 11, 2022 8:00 AM PDT
Date: October 11, 2022 Time: 8:00am (PDT), 11:00pm (EDT), 5:00pm (CEST) Multiomic profiling of cell populations at single-cell resolution is revolutionizing scientists’ understanding o...
MAR 29, 2018 1:30 PM PDT

Cannabidiol Treatment of Epilepsy and Autism Through Restoration of GABAergic Signaling



Show Resources
Loading Comments...
Show Resources