MAY 31, 2018 12:00 PM PDT

Cas9-Based Genome Engineering

C.E. CREDITS: P.A.C.E. CE | Florida CE
Speakers
  • Assistant Professor of Pathology & Cell Biology, Columbia University Irving Medical Center
    Biography
      Alejandro (Alex) Chavez, M.D., Ph.D. is an Assistant Professor of Pathology and Cell Biology at Columbia University. His research group strives to push the boundaries of genetic engineering by developing new methods with which to modify and regulate genomes. To date, his lab has generated methods for performing targeted gene activation or repression, developed some of the first synthetic inheritance-biasing elements (gene drives), created techniques for simultaneously generating hundreds of mutant cells, each with a programmed genetic alteration, and constrained evolution through the development of an in vivo mutation prevention system. Dr. Chavez applies these tools either within his own laboratory or in collaboration with others to gain fundamental biological insights with a particular focus towards understanding neurodegenerative diseases and cancer.

    Abstract:

    I will discuss three novel technologies that our laboratory has developed in recent years. In the first part of the talk, I will describe our work on engineering variants of the RNA-guided endonuclease, Cas9, for the selective control of gene expression. I will then share our method for endowing Cas9 with single-nucleotide specificity and describe how it can be used to generate an in vivo mutation prevention system. Finally, I will outline a novel technology for the simultaneous generation of hundreds of mutant cells, each with a defined genetic alteration (point mutation, deletion or insertion), as well as methods for tracking the fitness of all generated mutants within a single experiment.
     

    Learning Objectives: 

    1. Current methods for Cas9-based transcriptional activation
    2. Strategy to endow Cas9 with allele specificity
    3. Describe our novel approach to generating and tracking the fitness of hundreds of mutant cell lines within a single experiment


    Show Resources
    You May Also Like
    MAY 22, 2018 08:00 AM PDT
    C.E. CREDITS
    MAY 22, 2018 08:00 AM PDT
    DATE: May 22, 2018TIME: 08:00AM PDT The nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are closely related transcription factors that...
    JUN 20, 2018 10:00 AM EDT
    C.E. CREDITS
    JUN 20, 2018 10:00 AM EDT
    DATE: June 20, 2018TIME: 07:00AM PDT, 10:00AM EDTIntroducing GE’s New Lyo-StableTM service. Sepsis is one of the top challenges facing hospitals in terms of clinical outcomes...
    MAY 24, 2018 09:30 AM PDT
    C.E. CREDITS
    MAY 24, 2018 09:30 AM PDT
    DATE: May 24, 2018 TIME: 9:30PM PDT The current gold standard in in vitro pre-clinical cancer treatment screening remain cell lines,...
    MAY 03, 2018 11:00 AM PDT
    MAY 03, 2018 11:00 AM PDT
    DATE: May 3, 2018TIME: 11:00AM PDT, 2:00PM EDTWhile stress is one of the leading causes of neuropsychiatric disorders, the molecular underpinnings of how stress induces alterations in b...
    APR 27, 2018 10:00 AM PDT
    C.E. CREDITS
    APR 27, 2018 10:00 AM PDT
    DATE: April 27, 2018TIME: 10:00am PST, 1:00pm ESTGlioblastoma (GBM) and Medulloblastoma (MB) are the most common adult and paediatric brain tumours, both of which can have devastating c...
    JUN 05, 2018 06:30 AM PDT
    C.E. CREDITS
    JUN 05, 2018 06:30 AM PDT
    DATE: June 5, 2018TIME: 06:30AM PDT, 3:30PM CEST Viscous liquids are a challenge in every lab that deals with them. Depending on how viscous a liquid is, pipetting is either...
    Loading Comments...