Characterization of N-linked glycans with the Released Glycans Workflow of MassHunter Bioconfirm Software

Speaker
  • Application Scientist, Agilent Technologies
    Biography
      Rebecca Glaskin is an LC/MS Application Scientist at Agilent with a focus on BioPharma applications supporting the LC-Q/TOF and IM-QTOF platforms. Prior to joining Agilent, Rebecca received her Ph.D. in analytical chemistry from Indiana University in the lab of Professor David Clemmer. While there she designed and constructed home-built instruments, pushing the limits of the mobility resolution that can be obtained with a circular drift tube for the separation of biomolecules (peptides, proteins, carbohydrates, and metabolites). While there, she also studied hydrogen/deuterium exchange of proteins in the gas-phase as a function of time and pressure. Rebecca then went to Boston University as a Postdoctoral Associate in the lab of Professor Catherine Costello to develop a database containing collision cross section values for glycans, peptides, and glycopeptides utilizing Agilent Technologies 6560 IM-QTOF. This database can be used to determine how the collision cross section is altered with the addition of individual saccharide units. The trendlines obtained from this database will be used to predict collision cross sections for glycopeptides based on the conformation and structure of the specific glycoform.

    Abstract
    Monoclonal antibodies are heterogeneous molecules that include glycosylation variants, carboxyl truncation, and deamidation that require extensive analytical characterization. These molecules are one of the fastest growing classes in the biopharmaceutical industry. This presentation will discuss a complete workflow for intact monoclonal antibody analysis with the use of the 6545XT AdvanceBio LC/Q-TOF platform and automatic data processing with MassHunter BioConfirm software.

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