MAY 17, 2018 07:00 AM PDT

A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells

Speakers
  • R&D Scientist, Thermo Fisher Scientific
    Biography
      Maya has been an R&D Scientist with Thermo Fisher Scientific for about three years, focusing primarily on the development of novel tools for protein expression in insect cells. She has a strong background in cell culture, molecular biology, and protein expression. Maya has obtained a BSc in Molecular Biology from Sofia University "St. Kliment Ohridski" (Bulgaria) and an MSc in Medical Biotechnologies from Wageningen University (The Netherlands).

    Abstract:

    The Baculovirus Expression Vector System (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically-defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant lot-to-lot variability in terms of both cell growth and protein expression.  Here, we present the development of a novel Sf9-based Baculovirus expression system based on a high-density, chemically-defined culture medium, a high-expressing Sf9 cell line and expression enhancers that allow for the consistent production of recombinant proteins with two-fold or greater improvements in protein titers compared to traditional BEVS workflows.


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