MENU
MAY 17, 2018 7:00 AM PDT

A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells

Speaker

Abstract

The Baculovirus Expression Vector System (BEVS) is one of the major platforms for recombinant protein production. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically-defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant lot-to-lot variability in terms of both cell growth and protein expression.  Here, we present the development of a novel Sf9-based Baculovirus expression system based on a high-density, chemically-defined culture medium, a high-expressing Sf9 cell line and expression enhancers that allow for the consistent production of recombinant proteins with two-fold or greater improvements in protein titers compared to traditional BEVS workflows.


Show Resources
You May Also Like
MAY 11, 2021 10:00 AM PDT
C.E. CREDITS
MAY 11, 2021 10:00 AM PDT
Date: May 11, 2021 Time: 10:00zm PDT Your samples are some of the most valuable assets in the laboratory. After spending countless hours on extraction and preparation, your conclusions could...
SEP 14, 2021 7:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
C.E. CREDITS
SEP 14, 2021 7:00 AM PDT
Add to Calendar Select one of the following: iCal Google Calendar Outlook Calendar Yahoo Calendar
Date: September 14, 2021 Time: 7am PDT, 10am EDT, 4pm CEST A conventional thermal cycler has long been a commodity product in the lab and end-point PCR techniques can be completed almost wit...
JUN 09, 2021 7:00 AM PDT
C.E. CREDITS
JUN 09, 2021 7:00 AM PDT
Date: June 9, 2021 Time: 09 June 2021, 7am PDT, 10am EDT, 4pm CEST cells with dramatic implications on the validity of past cell culture related research. The fact that at least 509 cell lin...
MAR 16, 2021 10:00 AM PDT
C.E. CREDITS
MAR 16, 2021 10:00 AM PDT
Date: March 16, 2021 Time: 10:00am (PST) Scientific progress and breakthroughs today are often too expensive for most institutions to acquire. Each year, the National Institutes of Health (N...
JUN 08, 2021 9:00 AM PDT
JUN 08, 2021 9:00 AM PDT
Date: June 8, 2021 Time: 9:00am PDT Reducing the spread of highly infectious and deadly diseases within the population, vaccine development is crucial in saving millions of lives each year....
APR 21, 2021 10:00 AM PDT
C.E. CREDITS
APR 21, 2021 10:00 AM PDT
Date: April 21, 2021 Time: 10:00am (PDT), 1:00pm (EDT) Serological testing for SARS-CoV-2 has been steadily adopted into clinical practice over the course of this pandemic. In this webinar,...
MAY 17, 2018 7:00 AM PDT

A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells


Specialty

Biotechnology

Molecular Biology

Biochemistry

Immunology

Cell Biology

Cell Culture

Cancer Research

Antibodies

Microbiology

Cancer Therapeutics

Gene Expression

Genetics

Infectious Disease

Bioinformatics

Protein Chemistry

Geography

Asia34%

North America32%

Europe18%

South America10%

Oceania2%

Africa2%

Registration Source

Website Visitors77%

WAVE Newsletter Promotion16%

Email Promotions6%

Website Banners1%

Job Title

Student21%

Research Scientist15%

Scientist15%

Facility/Department Manager8%

Executive8%

Medical Laboratory Technician6%

Medical Doctor/Specialist6%

Educator/Faculty4%

Post Doc4%

Clinical Laboratory Scientist3%

Lab Management3%

Biologist2%

Genetic Counselor1%

Engineer1%

Veterinarian1%

Organization

Academic Institution25%

Biotech Company15%

Research Institute10%

Life Science Company8%

Medical School6%

Hospital4%

Medical Center3%

Contract Research Organization (Cro)2%

Industrial Company2%

Clinical Laboratory2%

Pharmaceutical Company2%

Non-Profit Organization2%

Media1%

Government1%

Other6%


Show Resources
Loading Comments...
Show Resources