JUL 31, 2019 9:00 AM PDT

Choroid Plexus Epithelial Cell 2D & Modified 3D Cell Culture Model

Speaker
  • Postdoctoral Fellow Department of Physiology, Louisiana State University Health Sciences Center
    Biography
      My passion for science started at a very young age. My dad was a forensics investigator for the New Orleans police department & after school I would spend time in the lab where he taught me about DNA, fingerprints, & microscopy. I was fascinated by the puzzle of all of it and I made my life goal to be an "ist" (scientist, biologist, paleontologist, etc.). My teachers & professors encouraged my love of science & made sure to emphasize the contributions of women in science, like Marie Curie, Rosalind Franklin, & Barbara McClintock. I admired the sacrifices these women made in order to help humanity & advance their fields.
      I received a scholarship to attend Randolph College where I received a bachelor's of science in Biology & Psychology with a pre-med concentration. There I learned the value of experiments & research from the best professors I have ever met, & was encouraged to pursue a career in research. I was then accepted to Tulane University School of Medicine where I received my PhD in Biomedical Sciences with a concentration in Microbiology/Immunology in 2019. I completed my degree at Tulane National Primate Research Center under the guidance of Dr. Marcelo Kuroda, Dr. Woong-Ki Kim, & Dr. Andrew MacLean. My dissertation focused on the neuroimmunology & neurovirology of HIV-Associated Neurocognitive Disorders in a simian model, with emphasis on macrophages of the central nervous system, the choroid plexus (blood-CSF barrier), & inflammaging. One of my key projects was the creation of a primary rhesus macaque choroid plexus cell culture for use studying the effects of inflammation on the blood-CSF barrier.
      Currently, I am a Postdoctoral Research Fellow at LSU Health Sciences Center where I work in the Physiology Department in Scott Edwards Lab studying the neurobiological interactions of alcohol, opioids, & pain in rat & simian models. In my free-time I love to spend time with my two dogs, Wishbone & Thor, & going on adventures to try new food with my husband.

    Abstract
    DATE:  July 31, 2019
    TIME:   9:00am PT, 12:00pm ET
     
    The choroid plexus, which makes up the blood-cerebrospinal fluid barrier in the central nervous system (CNS), lines the ventricles, produces cerebrospinal fluid, and protects the brain via a physical barrier. Perivascular macrophages line the stromal capillaries through it, and tight junctions between the apical sides of the epithelial cells regulate the microenvironment. The choroid plexus is also believed to be an immune interface between peripheral and CNS immune systems and plays a major role in the resolution of neuroinflammation by recruiting monocytes and leukocytes into the CNS. It has also been proposed to be a target of viral infection, such as a human immunodeficiency virus (HIV) reservoir, and a site of damage in cerebral hemorrhage, stroke, and hypoxia. Since the choroid plexus can allow transmigration of leukocytes, recruit myeloid cells, control diffusion of small molecules and water, and control drug permeability into the CSF, it is important to have an ex-vivo culture model.
     
    We designed a rhesus macaque 2D choroid plexus epithelial cell culture, as well as a modified 3D cell culture model, in order to study activation, diffusion, and migration through the choroid plexus. Our hope was to be able to understand the response of the blood-CSF barrier to peripheral HIV infection, as the rhesus macaque is an ideal animal model of infection. This model can also be used to study pro- and anti-inflammatory challenges, as well as barrier properties. 
     
     
    Learning Objectives:
    • To understand history, development, & function of the Blood-CSF Barrier
    • Understanding the pathology & therapeutic potential of the Choroid Plexus
    • Designing a 2D and modified 3D Choroid Plexus epithelial cell model system
     
     
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