Reliable expression of recombinant proteins in human or CHO cells is essential for many aspects of biomedical research and drug development but is often hampered by low expression yields that limit subsequent structural and functional analyses. The full portfolio of Thermo Fisher Scientific, from GeneArt™ gene synthesis to optimized mammalian expression systems, offers researchers the unique opportunity to perfect their gene expression results. Our proprietary sequence optimization algorithm GeneOptimizer™ enhances the sequence through a parallel multiparameter approach. To further maximize protein yield, you can choose from our best serum-free Gibco™ FreeStyle™ or Invitrogen™ Expi293™ and ExpiCHO™ expression systems. This holistic approach - from electronic gene sequence or accession number to the purified protein - is the most efficient way to exploit your gene-to-protein workflow for highest performance, either by purchasing reagents for internal work or outsourcing the entire workflow as a highly customized service to the GeneArt™ protein team. The presentation explains the basics of gene optimization and gene synthesis and how the combination with the best expression system produces unsurpassed expression reliability and protein yield.